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Merck
CN

SRP6420

Kallikrein-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

别名:

KLK1, KLKR, Klk6, hK1

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 27.9 kDa, observed mol wt 40-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Pro 19 is the predicted N-terminal.)
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biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 27.9 kDa, observed mol wt 40-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Pro 19 is the predicted N-terminal.)

packaging

pkg of 10 μg

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... KLK1(3816)

General description

Kallikrein-1 (KLK1) belongs to the peptidase S1 family and Kallikrein subfamily. KLK1 contains one peptidase S1 domain. KLK-1 preferential cleavage of Arg-|-Xaa bonds in small molecule substrates and also has highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa. Glandular Kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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全球贸易项目编号

货号GTIN
SRP6420-10UG04061837257780