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Merck
CN

T0438

单克隆抗-转化生长因子-β1 小鼠抗

clone 9016, purified immunoglobulin, lyophilized powder

别名:

抗-TGF-β1, CEDLAP, DPD1, 潜伏期相关肽

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关于此项目

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00802737
Conjugate:
unconjugated
Clone:
9016, monoclonal
Application:
ELISA, FACS, ICC, IHC, Neutral, WB
Citations:
21

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9016, monoclonal

form

lyophilized powder

species reactivity

mouse, human, rat

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, neutralization: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P01137

application(s)

research pathology

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)

General description

转化生长因子-β1（TGFβ1）被定位到人类染色体19q13.2。
转化生长因子-β（TGF-β）属于分泌蛋白的TGF-β超家族。TGF-β 1以TGF-β1、TGF-β2和TGF-β3三种亚型存在。TGF-β家族参与多种信号通路，涉及丝氨酸/苏氨酸蛋白激酶。TGF-β通过与哺乳动物中的三种跨膜受体（I型，II型和III型受体）结合而起作用。 TGF-β调节的重要通路是SMAD、BMP、MAPK和PI3K/Akt通路。TGF-β 1参与细胞和组织的分化、增殖、炎症、单核细胞、中性粒细胞的迁移和趋化。TGF-β信号传导失调与许多炎症和自身免疫性疾病相关。抗转化生长因子-β1单克隆抗体与人、小鼠和大鼠TGF-β1特异性反应。在ELISA中，该抗体与TGF-β3和TGF-β5的交叉反应性低于2%。

Immunogen

中国仓鼠卵巢细胞系CHO衍生的重组TGF-β1和潜在TGF-β1

Application

推荐浓度：
蛋白质印迹法1 ug/mL（仅在非还原条件下）
免疫组化 3-25 μg/mL
流式细胞术 2.5 μg/106 细胞
ELISA捕获 2-8 μg/mL
ELISA检测 0.1-0.4 μg/mL
中和 0.3-1.0 μg/mL
小鼠源抗转化生长因子-β1单克隆抗体可用于：
  • 免疫组织化学检测平滑肌（2）和肝脏切片中的TGFβ1
  • 脑标本的蛋白质印迹和ELISA
  • 免疫印迹检测巨噬细胞中的TGFβ1

Biochem/physiol Actions

通过捕获ELISA，观察到与TGF-β3和TGF-β5的<2%交叉反应性。

Physical form

从含碳水化合物的磷酸盐缓冲液中（经0.2 μm过滤）冻干。

Preparation Note

以0.5 mg/mL的浓度在无菌PBS中复溶。

Other Notes

从杂交瘤培养上清液中纯化的蛋白A或G。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBX6210
SLBW5525
SLBS0285V
SLBQ2671V
SLBN9788V

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used to neutralize the biological activity of TGF-ß1?

    Yes, this antibody will neutralize TGF-ß1.  

  7. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used for immunohistochemistry with frozen and/or formalin-fixed paraffin-embedded sections?

    This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.

  8. Will Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, work for intracellular flow cytometry?

    This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.

  9. How is Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, different than T0688,  Anti-Transforming Growth Factor-?1 antibody produced in chicken?

    These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.

  10. What is the molecular weight of Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse,?

    TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4.
Fayed MR, et al.
Drug Discoveries & Therapeutics, 12(2), 58-67 (2018)
Gangwen Han et al.
International journal of biological sciences, 8(2), 228-235 (2012-01-19)
TGFβ1 was initially identified as a potent chemotactic cytokine to initiate inflammation, but the autoimmune phenotype seen in TGFβ1 knockout mice reversed the dogma of TGFβ1 being a pro-inflammatory cytokine to predominantly an immune suppressor. The discovery of the role
Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
Di Pardo A, et al.
Molecular Brain, 6(1), 55-55 (2013)
Alba Di Pardo et al.
Molecular brain, 6, 55-55 (2013-12-18)
Huntington Disease (HD) is a neurodegenerative disorder resulting from the expansion of polyglutamine stretch in the huntingtin protein (Htt). Mutant HTT (mHtt) leads to progressive impairment of several molecular pathways that have been linked to disease pathogenesis. Defects in the
The transforming growth factor-beta1 (TGFB1) gene is associated with chronic obstructive pulmonary disease (COPD).
Celedon JC, et al.
Human Molecular Genetics, 13(15), 1649-1656 (2004)
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Data Sheet - T0438

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