Anti-Phosphotyrosine (pY-256) antibody produced in rabbit

Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Phosphotyrosines have also been linked to other cellular functions. For instance, tyrosine 256 phosphorylation in atypical protein kinase C enables its entry into the nucleus of cells . Anti-Phosphotyrosine pTyr²⁵⁶ binds to tyrosine phosphorylated proteins. In immunoblotting, the antibody detects [pTyr256] in random peptides and phospho-L-tyrosine, but does not react with phospho-L-serine, phospho-L-threonine and L-tyrosine.

phospho-L-tyrosine containing random peptide pY-256, conjugated to KLH.

Anti-Phosphotyrosine (pY-256) antibody is suitable for use in western blot (1:1000) . It may also be used in indirect immunofluorescence (5-10 μg/mL using A431 stiμLated by human EGF), immunoblotting and immunocytochemistry.

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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