生物来源
human
方案
≥97% (SDS-PAGE and N-terminal analysis)
表单
lyophilized powder
效能
0.04-0.3 ng/mL ED50/EC50
质量
endotoxin tested
比活
1 × 106 U/mg
分子量
protein 25 kDa
包装
pkg of 1 μg
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤1 EU/μgtested (LAL test)
UniProt登记号
储存温度
−20°C
基因信息
human ... TGFB1(7040)
一般描述
The biologically active form of TGF-β1 (transforming growth factor-β1 human) exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3. TGF-β1 encodes a 25 kDa peptide. TGF-β1 precursor polypeptide is characterized with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The TGF-β1 gene is mapped to human chromosome 19q13.2.
应用
Transforming Growth Factor-β1 from human platelets has been used
- to study the induction of a-smooth muscle actin by transforming growth factor-1 (TGF-β1) in breast cancer cells.
- to study the efficiency of TGF-β1 in promoting embryonic stem-like cell proliferation with or without feeders.
- as control to analyse the isolated TGF-β1 HPLC using reverse-phase chromatography.
- to induce profibrotic response in renal fibroblasts and further study functional role for Smad3 (mothers against decapentaplegic homolog 3) T388 phosphorylation.
生化/生理作用
TGF-β1 plays a key role in embryogenesis and tissue homeostasis. TGF-β1 is fundamentally involved in differentiation by mediating adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, migration, death, tissue growth, and other functions in a wide range of cell types. TGF-β1 is associated with the developmental and metastasis of colorectal cancer. Downregulation of TGF-β1 might lead to many disease conditions, including tumor formation.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
外形
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% TFA, containing 50 ug of BSA per 1 ug cytokine
分析说明
The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
此项目有
TGF β signalling and its role in tumour pathogenesis.
Kaminska B, et al.
Acta Biochimica Polonica, 52.2, 329-329 (2005)
Epithelial to Mesenchymal Transition in Human Breast Cancer Can Provide a Nonmalignant Stroma
Petersen OW, et al.
The American Journal of Pathology, 162(2), 391?402-391?402 (2003)
Regulation of Renal Fibrosis by Smad3 Thr388 Phosphorylation
Xinli Qu, et al.
The American Journal of Pathology, 184, 944-952 (2014)
In vitro culture of embryonic disc cells from porcine blastocysts.
Hochereau-de Reviers MT and Perreau C
Reproduction, Nutrition, Development, 33.5, 475-483 (1993)
Aaron Schindeler et al.
BMC musculoskeletal disorders, 11, 105-105 (2010-06-01)
Transforming growth factor-beta (TGF-beta) and bone morphogenetic proteins (BMPs) utilize parallel and related signaling pathways, however the interaction between these pathways in bone remains unclear. TGF-beta inhibition has been previously reported to promote osteogenic differentiation in vitro, suggesting it may
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