T1654
Transforming Growth Factor-β1 来源于人类血小板
TGF-β1, lyophilized powder, suitable for cell culture, 1 × 106 U/mg
别名:
TGF-β1
biological source
human
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
0.04-0.3 ng/mL ED50/EC50
quality
endotoxin tested
specific activity
1 × 106 U/mg
mol wt
protein 25 kDa
packaging
pkg of 1 μg, pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μgtested (LAL test)
UniProt accession no.
storage temp.
−20°C
General description
The biologically active form of TGF-β1 (transforming growth factor-β1 human) exists as a disulphide-linked homodimer. In mammals, three isoforms of TGF-β has been identified : TGF-β1, TGF-β2 and TGF-β3. TGF-β1 encodes a 25 kDa peptide. TGF-β1 precursor polypeptide is characterized with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The TGF-β1 gene is mapped to human chromosome 19q13.2.
Application
Transforming Growth Factor-β1 from human platelets has been used
- to study the induction of a-smooth muscle actin by transforming growth factor-1 (TGF-β1) in breast cancer cells.
- to study the efficiency of TGF-β1 in promoting embryonic stem-like cell proliferation with or without feeders.
- as control to analyse the isolated TGF-β1 HPLC using reverse-phase chromatography.
- to induce profibrotic response in renal fibroblasts and further study functional role for Smad3 (mothers against decapentaplegic homolog 3) T388 phosphorylation.
Biochem/physiol Actions
TGF-β1 plays a key role in embryogenesis and tissue homeostasis. TGF-β1 is fundamentally involved in differentiation by mediating adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, migration, death, tissue growth, and other functions in a wide range of cell types. TGF-β1 is associated with the developmental and metastasis of colorectal cancer. Downregulation of TGF-β1 might lead to many disease conditions, including tumor formation.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.
Physical form
Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% TFA, containing 50 ug of BSA per 1 ug cytokine
Analysis Note
The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
The transforming growth factor-β1 (TGFB1) gene is associated with chronic obstructive pulmonary disease (COPD)
Celedon JC, et al.
Human Molecular Genetics, 13, 1649?1656-1649?1656 (2004)
TGF β signalling and its role in tumour pathogenesis.
Kaminska B, et al.
Acta Biochimica Polonica, 52.2, 329-329 (2005)
Epithelial to Mesenchymal Transition in Human Breast Cancer Can Provide a Nonmalignant Stroma
Petersen OW, et al.
The American Journal of Pathology, 162(2), 391?402-391?402 (2003)
Regulation of Renal Fibrosis by Smad3 Thr388 Phosphorylation
Xinli Qu, et al.
The American Journal of Pathology, 184, 944-952 (2014)
Aaron Schindeler et al.
BMC musculoskeletal disorders, 11, 105-105 (2010-06-01)
Transforming growth factor-beta (TGF-beta) and bone morphogenetic proteins (BMPs) utilize parallel and related signaling pathways, however the interaction between these pathways in bone remains unclear. TGF-beta inhibition has been previously reported to promote osteogenic differentiation in vitro, suggesting it may
相关内容
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持