T2827
Monoclonal Anti- dTAF80 小鼠抗
clone 3D10, purified from hybridoma cell culture
别名:
Anti- TAF5, Anti- TAF80/85, Anti- TAFII80, Anti- dmTAF5
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
3D10, monoclonal
Application:
ELISA (i), WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
3D10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~80 kDa
species reactivity
Drosophila melanogaster
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
indirect ELISA: suitable, western blot: 8-16 μg/mL using nuclear extracts of D.Mel cells.
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
Drosophila melanogaster ... Taf5(47900)
General description
Monoclonal Anti-dTAF80 (mouse IgG2b isotype) is derived from the hybridoma 3D10 produced by the fusion of mouse myeloma cells and splenocytes from mice. Drosophila tightly associated factor 80 (dTAF80), also known as tightly associated factor 5 (TAF5) consists of numerous copies ofβ−transducin (WD40) repeat.
Immunogen
Drosophila TFIID complex.
Application
Anti-dTAF80 antibody, mouse monoclonal may be used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Drosophila tightly associated factor 80 (dTAF80) recognizes and interacts with a higher-order complex containing TATA box-binding protein (TBP), delta N250, 110 and 60.
Monoclonal Anti-dTAF80 reacts specifically with Drosophila dTAF80
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Preparation Note
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples shouldbe discard if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
此项目有
The dTAF II 80 subunit of Drosophila TFIID contains beta-transducin repeats
Dynlacht B D, et al.
Nature, 363(6425), 176-179 (1993)
Molecular cloning of a novel 120-kDa TBP-interacting protein
Yogosawa S, et al.
Biochemical and Biophysical Research Communications, 229(2), 612-617 (1996)
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