T6200
凝血酶 来源于牛血浆
lyophilized powder, 40-300 NIH units/mg protein (biuret)
别名:
IIa 因子
form
lyophilized powder
specific activity
40-300 NIH units/mg protein (biuret)
composition
Protein, 40-60%
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... F2(280685)
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General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Lyophilized powder containing sucrose, sodium chloride and Tris.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess characterization of platelet dysfunction after trauma.
Biochem/physiol Actions
丝氨酸蛋白酶，选择性断开纤维蛋白原中的精氨酸-甘氨酸键，形成纤维蛋白以及纤维蛋白肽 A 和 B。
Physical form
Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0
Preparation Note
Prepared using bovine lung thromboplastin for conversion to the active form.
Analysis Note
基于 Biggs 改性法的 NIH 分析过程使用 0.2mL 稀释血浆（由血浆与等量生理盐水混合制得）作为底物，以及 0.1mL 凝血酶样品（在含 1% 白蛋白缓冲液中保持稳定）。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。
活性是通过与一种K批次的NIH凝血酶参考标准品进行直接比较而得到并以NIH单位进行表达的。
Other Notes
查看更多关于 凝血酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Matthew E Kutcher et al.
The journal of trauma and acute care surgery, 73(1), 13-19 (2012-06-30)
The increased morbidity and mortality associated with coagulopathy and thrombocytopenia after trauma are well described. However, few studies have assessed platelet function after injury. Blood samples were prospectively collected from 101 patients with critical injury and trauma on arrival to
Response.
Sarah T Garber et al.
Journal of neurosurgery, 118(2), 485-485 (2013-03-16)
The reply.
Peter R Kowey et al.
The American journal of medicine, 126(4), e23-e23 (2013-03-20)
Stephen R Clark et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(15), 5875-5880 (2013-03-27)
Aminophospholipid (APL) trafficking across the plasma membrane is a key event in cell activation, apoptosis, and aging and is required for clearance of dying cells and coagulation. Currently the phospholipid molecular species externalized are unknown. Using a lipidomic method, we
Hans P Kohler
Blood, 121(11), 1931-1932 (2013-03-16)
In this issue of Blood, Smith and colleagues report on the functional role of the interaction between these 2 proteins by studying the involved binding sites responsible for clot stabilization.(1)
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