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Merck
CN

T6454

Anti-Timeless (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-TIM, Anti-TIM1, Anti-Timeless1, Anti-hTIM

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关于此项目

NACRES:
NA.77
UNSPSC Code:
12352202
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
3

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~160 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cells over expressing human Timeless, indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human Timeless, western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human Timeless

UniProt accession no.

Q9UNS1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TIMELESS(8914)

General description

The gene TIMELESS is located on the human chromosome at 12q13.3. The encoded protein is a component of the replisome complex.

Application

Anti-Timeless (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Anti-Timeless (C-terminal) recognizes human Timeless (also known as hTIM, TIM, TIM1, Timelesss1).
Timeless (Tim) forms a complex with Tim interacting protein (Tipin), found by two-hybrid screening. It associates with components of the replication fork complex through direct binding with the (RPA) and are important for efficient DNA synthesis. Timeless plays a role in replication checkpoint responses to genotoxic stress. It also plays an important role in the maintenance of genome stability during unperturbed DNA replication. Overexpression of the TIMELESS gene is associated with cervical cancer. Annihilation of this gene induces apoptosis and cell senescence.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
029K4775

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Timeless maintains genomic stability and suppresses sister chromatid exchange during unperturbed DNA replication
Urtishak K A, et al.
The Journal of biological chemistry, 284(13), 8777-8785 (2009)
Aberrantly Expressed Timeless Regulates Cell Proliferation and Cisplatin Efficacy in Cervical Cancer
Zhou J, et al.
Human Gene Therapy, 31(5-6), 385-395 (2020)
The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement
Unsal KK, et al.
Molecular and Cellular Biology, 27(8), 3131-3142 (2007)

相关内容

Data Sheet - T6454

Instructions

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