form
lyophilized powder
extent of labeling
18-31 μmol per mL
matrix active group
hydroxypropyl 2-pyridyl disulfide
swelling
1 g swells to 4-5 mL gel
storage temp.
2-8°C
Application
Thiopropyl Sepharose™ 6B is used in protein chromatography, affinity chromatography and hydrophobic interactions. Thiopropyl Sepharose™ 6B has been used for the removal of inhibitors from PCR extracts from evidence collected for law enforcement murder investigations. Thiopropyl Sepharose™ 6B has also been used to describe a method for the investigation of functional properties of distinct domains of viral thiol proteins, including the influenza virus membrane M1 protein.
Physical form
Lyophilized powder stabilized with lactose and dextran
Legal Information
Sepharose is a trademark of Cytiva
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Michael T Forrester et al.
Journal of lipid research, 52(2), 393-398 (2010-11-04)
Protein S-acylation is a major posttranslational modification whereby a cysteine thiol is converted to a thioester. A prototype is S-palmitoylation (fatty acylation), in which a protein undergoes acylation with a hydrophobic 16 carbon lipid chain. Although this modification is a
Markandeswar Panda et al.
Journal of chromatography. A, 1202(1), 75-82 (2008-07-08)
Green fluorescent protein (GFP) fused to the C-terminal 100 amino acids of CAAT enhancer binding protein (C/EBP) also containing an N-terminal (His)(6) tag (GFP-C/EBP) was used as a transcription factor model to test whether thiol-disulfide exchange reactions could be used
F Desarnaud et al.
Journal of chromatography, 603(1-2), 95-104 (1992-06-19)
The major problem usually encountered in the application of the (strept)avidin-biotin system to the purification of proteins (or other biological molecules) lies in the difficult reversion of the interaction between immobilized (strept)avidin and the adsorbed biotinylated protein. Among the proposed