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Merck
CN

T9650

Sigma-Aldrich

TRIS醋酸盐-EDTA缓冲液

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

别名:

TAE缓冲液

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About This Item

MDL编号:
UNSPSC代码:
41105319
PubChem化学物质编号:
NACRES:
NA.25

等级

for molecular biology

质量水平

无菌性

non-sterile; 0.2 μm filtered

产品线

BioReagent

形式

solution

适用性

suitable for gel electrophoresis (after dilution to working concentration)

异质活性

Protease, none detected
RNAse, none detected

SMILES字符串

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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应用

Tris乙酸盐-EDTA缓冲液已被用于在DNA琼脂糖凝胶电泳过程中制备琼脂糖凝胶。
TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液,也可用于非变性RNA琼脂糖凝胶电泳。 双链DNA在TAE中比在其他缓冲液中运行更快,但也可能在延长的电泳过程中耗尽。 在延长的电泳过程中,缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。 浓缩TAE缓冲液稀释后得到1× TAE缓冲液,加入40 mM醋酸三酯和1 mM EDTA,pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。 为了达到最大的分辨率,推荐电压小于5V/cm(电极单元间距离)。

其他说明

0.4M Tris 乙酸盐,pH 约为 8.3,含有 0.01M EDTA。

制备说明

用 18 兆欧水制备的溶液
由经过生物技术性能认证的Trizma碱(T6066)和分子生物学试剂EDTA二钠盐(E5134)制备而成。溶液也含有醋酸(A6283);粉状混合物含Trizma醋酸(T1258)。

象形图

Health hazard

警示用语:

Warning

危险声明

预防措施声明

危险分类

STOT RE 2 Inhalation

靶器官

Respiratory Tract

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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访问文档库

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
Quan J and Tian J
Nature Protocols, 6(2), 242-242 (2011)
Yuksel Temiz et al.
Scientific reports, 8(1), 10603-10603 (2018-07-15)
The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for
Joseph P Torella et al.
Nature protocols, 9(9), 2075-2089 (2014-08-08)
Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts, and they hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these

实验方案

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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