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Merck
CN

T9702

Anti-p8/TTD-A (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB
Citations:
4

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa (tagged fused protein)

species reactivity

mouse, rat, human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human p8/TTD-A, western blot: 1-2 μg/mL using HEK-293T over-expressing human p8/TTD-A

UniProt accession no.

Q6ZYL4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GTF2H5(404672)
mouse ... Gtf2H5(66467)
rat ... Gtf2h5(502227)

General description

8-kDa trichothiodystrophy group A (p8/TTD-A) protein is the tenth subunit of general transcription factor IIH (TGFIIH). This protein exists as attached to transcription factor IIH (TFIIH) and as a free fraction that alternates between the cytoplasm and nucleus. p8/TTD-A gene is mapped on the human chromosome at 6q25.3.
p8/TTD-A (C-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

Application

Anti-p8/TTD-A (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

8-kDa trichothiodystrophy group A (p8/TTD-A) protein defects leads to a sun-sensitive form of trichothiodystrophy (TTD), a rare autosomal recessive disorder. It is essential for the stability of the transcription factor IIH (TFIIH) complex. The cells from patients with p8/TTD-A have reduced levels of TFIIH while overexpressing p8/TTD-A restored the cellular level TFIIH complex.
Anti-p8/TTD-A (C-terminal) specifically recognizes human, rat, and mouse p8/TTD-A.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
108K4770
108K4771

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p8/TTD-A as a repair-specific TFIIH subunit
Coin F, et al.
Molecular Cell, 21(2), 215-226 (2006)
Dynamic interaction of TTDA with TFIIH is stabilized by nucleotide excision repair in living cells
Giglia-Mari G, et al.
PLoS Biology, 4(6), e156-e156 (2006)
Correlation between 6q25. 3 deletion status and survival in pediatric intracranial ependymomas
Monoranu C M, et al.
Cancer Genetics and Cytogenetics, 182(1), 18-26 (2008)
Sublimiting concentration of TFIIH transcription/DNA repair factor causes TTD-A trichothiodystrophy disorder
Vermeulen W, et al.
Nature Genetics, 26(3), 307-313 (2000)

相关内容

Data Sheet - T9702

Instructions

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