产品名称
总膳食纤维测定试剂盒, sufficient for ~100 assays
usage
sufficient for ~100 assays
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Quality Level
Application
此检测通过酶促和比重联合法来测定食物中的总膳食纤维含量。 先使用热稳定的α-淀粉酶对干燥的脱脂食物样品进行凝胶化，然后通过蛋白酶和淀粉葡糖苷酶进行酶促消化，以除去样品中存在的蛋白质和淀粉。 之后加入乙醇来沉淀可溶性的膳食纤维。 过滤剩余物质，并使用乙醇和丙酮进行洗涤。 干燥后对剩余物质进行称重。 一半样品进行蛋白质分析，其余进行灰化。 总膳食纤维为扣除蛋白质和灰的重量之后的残余物质重量。
Biochem/physiol Actions
使用酶促和比重分析联合方法。 本操作步骤基于已发表的官方分析方法(Official Methods of Analysis)，15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990) 。
Other Notes
检测总膳食纤维的试剂。
仅试剂盒组分
产品编号
说明
- α-Amylase, Heat-stable 10 mL
- Amyloglucosidase 10 mL
- Celite 50 g
- Protease 500 mg
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
target_organs
Lungs, Respiratory system
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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Lucile J M Martin et al.
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