Merck
CN

TDF100A

Supelco

总膳食纤维测定试剂盒

sufficient for ~100 assays

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NACRES:
NA.84

用途

sufficient for ~100 assays

质量水平

技术

photometry: suitable

应用

food and beverages
general analytical

储存温度

2-8°C

应用

此检测通过酶促和比重联合法来测定食物中的总膳食纤维含量。 先使用热稳定的α-淀粉酶对干燥的脱脂食物样品进行凝胶化,然后通过蛋白酶和淀粉葡糖苷酶进行酶促消化,以除去样品中存在的蛋白质和淀粉。 之后加入乙醇来沉淀可溶性的膳食纤维。 过滤剩余物质,并使用乙醇和丙酮进行洗涤。 干燥后对剩余物质进行称重。 一半样品进行蛋白质分析,其余进行灰化。 总膳食纤维为扣除蛋白质和灰的重量之后的残余物质重量。

其他说明

检测总膳食纤维的试剂。

原理

使用酶促和比重分析联合方法。 本操作步骤基于已发表的官方分析方法(Official Methods of Analysis),15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990) 。

仅试剂盒组分

产品编号
说明

  • α-Amylase, Heat-stable 10 mL

  • Amyloglucosidase 10 mL

  • Celite 50 g

  • Protease 500 mg

相关产品

产品编号
说明
价格

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

靶器官

Lungs, Respiratory system

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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The effects of air drying temperature on dietary fibre, texture and microstructure of the Cape gooseberry fruits during convective dehydration in the range of 50-90 ºC were investigated. The ratio of insoluble dietary fibre to soluble dietary fibre was higher
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Journal of food science, 82(9), 2041-2050 (2017-07-29)
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