U5258
Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-UCH-L1
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
species reactivity
human, mouse, rat
technique(s)
microarray: suitable, western blot: 1:1,000-1:2,000 using cytosolic fraction (S1) of mouse brain or whole cell extract of human lung carcinoma A549 cell line, western blot: 1:5,000-1:10,000 using cytosolic fraction (S1) of rat brain
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UCHL1(7345)
mouse ... Uchl1(22223)
rat ... Uchl1(29545)
General description
Ubiquitin C-terminal hydrolase L1 (UCH-L1) is encoded by the gene mapped to human chromosome 4p13. It is a 223 amino acid deubiquitinating enzyme, expressed highly in neurons.
Immunogen
synthetic peptide corresponding to amino acids 202-216 located near the C-terminus of rat UCH-L1, conjugated to KLH. This sequence is identical in human, mouse, bovine, porcine, and guinea pig UCH-L1. No homology is found with other known UCH-L isoforms.
Application
Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit has been used in immunoblotting.
Biochem/physiol Actions
Ubiquitin C-terminal hydrolase L1 (UCH-L1) hydrolyze C-terminal ubiquityl esters and amides in vitro, which is an essential reaction during cytoplasmic protein degradation. peptide-ubiquityl amides are the most favorable substrates. UCH-L1 also acts as a ubiquitin (Ub) ligase. Mutation in the gene is associated with the development of neurodegenerative diseases, such as Parkinson′s disease, spinocerebellar ataxia (SCA) and Huntington′s disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Reduced expression of the G209A alpha-synuclein allele in familial parkinsonism
Markopoulou K, et al.
Annals of Neurology, 46(3), 374-381 (1999)
The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility
Liu Y, et al.
Cell, 111(2), 209-218 (2002)
Krishnan Sriram et al.
Toxicology and applied pharmacology, 449, 116137-116137 (2022-06-25)
Workers in the oil and gas industry are at risk for exposure to a number of physical and chemical hazards at the workplace. Chemical hazard risks include inhalation of crude oil or its volatile components. While several studies have investigated
Reversible monoubiquitination regulates the Parkinson disease-associated ubiquitin hydrolase UCH-L1.
Robin K Meray et al.
The Journal of biological chemistry, 282(14), 10567-10575 (2007-01-30)
Deubiquitinating enzymes (DUBs) are negative regulators of protein ubiquitination and play an important role in ubiquitin-dependent processes. Recent studies have found that diverse cellular mechanisms are employed to control the activity of DUBs. Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is a highly
Karnam Shruthi et al.
Journal of cellular biochemistry, 120(4), 5962-5973 (2018-10-15)
The ubiquitin-proteasome system (UPS) has been implicated in the pathogenesis of many neurodegenerative diseases. Endoplasmic reticulum (ER) stress is shown to play a pathological role in the development of diabetes and its complications. Hence, the current study is aimed to
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