recombinant
expressed in baculovirus infected Sf9 cells
assay
≥98% (SDS-PAGE)
form
solution
mol wt
20 kDa
shipped in
dry ice
storage temp.
−70°C
Application
Typical enzyme concentration to support in vitro conjugation is 100nM to 1μM dependent upon conditions.
Biochem/physiol Actions
UbcH9 mediates the conjugation of SUMO-1, SUMO-2, and SUMO-3 to a diverse variety of proteins without the help of an E3 ligase. This E2 conjugating enzyme interacts directly with protein substrates that are modified by SUMOylation, and may help in substrate recognition. UbcH9 is specific for the conjugation of proteins modified by SUMOylation.
Physical form
Supplied at a concentration of 0.5 μg/μl in a solution of 50mM HEPES, pH 7.6, 125 mM NaCl, 10% glycerol, and 1 mM DTT.
aqueous glycerol solution
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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