生物来源
rabbit
表单
solution
技术
ligand binding assay: suitable
运输
dry ice
储存温度
−70°C
一般描述
Manufactured for Sigma by Boston Biochem., Inc.
应用
Ubiquitin Conjugating Enzyme Fractions mammalian may be used in transferring the activated ubiquitin from E1 to the substrate through an additional high energy thiol ester intermediate E2-S-ubiquitin.
生化/生理作用
Full complement of purified conjugation enzymes (E1, E2s, and E3s) that are found in mammalian Fraction II.
Ubiquitin Conjugating Enzyme Fractions mammalian signifies the full complement of purified conjugation enzymes (E1, E2s, and E3s), which are found in mammalian Fraction II. Ubiquitin conjugating enzyme facilitates the stimulation of IκB kinase complex by TRAF6. It also assists in assembly of novel polyubiquitin chains for DNA repair.
储存分类代码
10 - Combustible liquids
法规信息
新产品
此项目有
L Deng et al.
Cell, 103(2), 351-361 (2000-11-01)
TRAF6 is a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. We have purified a heterodimeric protein complex that links TRAF6 to IKK activation. Peptide mass fingerprinting analysis reveals that this complex
R M Hofmann et al.
Cell, 96(5), 645-653 (1999-03-25)
Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromeric
D Ganoth et al.
The Journal of biological chemistry, 263(25), 12412-12419 (1988-09-05)
It was found previously that proteins conjugated to ubiquitin are degraded by an ATP-dependent enzyme system, but the mode of action of this system was unknown. We have resolved from reticulocyte extracts three factors that are required for the ATP-dependent
A Hershko et al.
The Journal of biological chemistry, 258(13), 8206-8214 (1983-07-10)
By affinity chromatography of a crude reticulocyte extract on ubiquitin-Sepharose, three enzymes required for the conjugation of ubiquitin with proteins have been isolated. One is the ubiquitin-activating enzyme (E1), which is covalently linked to the affinity column in the presence
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