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安全信息

UPS2

Sigma-Aldrich

蛋白质组学动态范围标准品套装

Protein Mass Spectrometry Calibration Standard

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别名:
UPS, UPS1, abrf, abrf sprg, abrf standard, abrf standards, human protein, human proteins, lc mass spectrometry, lc ms, lcms, ltq, mass spec, mass spec standards, mass spectrometers, mass spectrometry proteomics, orbi-trap, protein standard, protein standards, proteomic, proteomics analysis, proteomics mass spectrometry, proteomics protein, proteomics research, quadrupole, sprg, sprg standard, standard, standards, standards for mass spec, tandem mass spectrometry, universal proteomics, universal proteomics standard
EC 号:
232-650-8
NACRES:
NA.24

生物来源

human

形式

ready-to-use solution

质量

Protein Mass Spectrometry Calibration Standard

浓度

10.6 μg/ampule protein

technique(s)

mass spectrometry (MS): suitable

运输

wet ice

储存温度

−20°C

相关类别

一般描述

蛋白质组学动态范围标准品套装由48种单独的人源或人序列重组蛋白的混合物产生，每种重组蛋白都被选择用于限制异源翻译后修饰（PTM）。蛋白质标准品由6种蛋白质的6种混合物配制而成，其动态范围为5个数量级，范围为50皮摩尔至500摩尔。在配制之前，通过氨基酸分析（AAA）对每种蛋白质进行定量。

应用

蛋白组学动态范围标准套件用于基于强度的绝对定量（iBAQ），适用 大肠杆菌 蛋白、 胚胎干细胞（ESC）和神经元前体细胞（NPC）蛋白组。 也作为标准品，无标记定量刺突 HeLa 细胞。
蛋白质组学动态范围标准品套装可用于在分析复杂蛋白质样品之前标准化和/或评估质谱（例如，LC-MS / MS，MALDI-TOF-MS等）和电泳分析条件。UPS2 可用于在已知复杂标准样品的运行之间交叉包含宝贵的实验数据集。从而确认分析方法的耐用性和所用仪器的稳定性。此外，生成或比较来自定义不明确的样品的质谱数据的实验室可以使用UPS2作为外部参考，以协助评估结果和实验方法。
蛋白质组学动态范围标准品套装已用于在 Orbitrap 分析仪上使用全离子碎片对动态范围通用蛋白质标准品进行定量。它已被用作LC-MS（液相色谱 - 质谱）/ MS分析中基于强度的蛋白质绝对定量（iBAQ）的标准。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg化学品安全说明书

警示用语：

Danger

危险分类

Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--人源产品

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示例

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L Arike et al.
Journal of proteomics, 75(17), 5437-5448 (2012-07-10)
Three different label-free proteome quantification methods--APEX, emPAI and iBAQ--were evaluated to measure proteome-wide protein concentrations in the cell. All the methods were applied to a sample from Escherichia coli chemostat culture. A Pearson squared correlation of approximately 0.6 among the
Jürgen Cox et al.
Molecular & cellular proteomics : MCP, 13(9), 2513-2526 (2014-06-20)
Protein quantification without isotopic labels has been a long-standing interest in the proteomics field. However, accurate and robust proteome-wide quantification with label-free approaches remains a challenge. We developed a new intensity determination and normalization procedure called MaxLFQ that is fully
Mari J Aaltonen et al.
The Journal of cell biology, 213(5), 525-534 (2016-06-01)
Mitochondria exert critical functions in cellular lipid metabolism and promote the synthesis of major constituents of cellular membranes, such as phosphatidylethanolamine (PE) and phosphatidylcholine. Here, we demonstrate that the phosphatidylserine decarboxylase Psd1, located in the inner mitochondrial membrane, promotes mitochondrial
Björn Schwanhäusser et al.
Nature, 473(7347), 337-342 (2011-05-20)
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale.
Taejoon Kwon et al.
Journal of proteome research, 10(7), 2949-2958 (2011-04-15)
Shotgun proteomics using mass spectrometry is a powerful method for protein identification but suffers limited sensitivity in complex samples. Integrating peptide identifications from multiple database search engines is a promising strategy to increase the number of peptide identifications and reduce
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Universal Proteomics Standards – Validating the Future of Proteomics

The era of high-throughput proteomics has recently blossomed due in large part to advances in the methods by which proteins and proteomes are analyzed. Improved fractionation techniques, combined with advances in mass spectrometry, have decreased concerns of sample complexity, and directed more focus towards high-throughput techniques.

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UPS1 & UPS2 Proteomic Standards

Standardize Your Research. We offer both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry.

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