Monoclonal Anti-WIPI-1 antibody produced in mouse

Monoclonal Anti-WIPI-1 (mouse IgG1 isotype) is derived from the hybridoma WIPI1-4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human WIPI-1, conjugated to KLH. WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat (Trp-Asp) proteins. WIPI-1 has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is expressed in normal human tissues with high levels in skeletal muscle, heart and testis.

Monoclonal Anti-WIPI-1 antibody has been used in immunoblotting, immunoprecipitation.

WIPI-1 (WD repeat domain phosphoinositide-interacting protein 1) plays an important role in signal transduction, transcription regulation and apoptosis. Endogenous WIPI-1 partially colocalizes with the autophagosomal marker LC3 and induction of autophagy leads to the accumulation of WIPI-1 in large vesicular and cup-shaped structures that are characteristic for autophagy-linked proteins. WIPI-1 is aberrantly expressed in human cancer and is linked to starvation induced autophagy.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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