biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
rat, human, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates, indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells, western blot: 0.5-1 μg/mL using PC12 cell lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)
General description
Y-box-结合蛋白(YB-1)也称为核酸酶敏感元件结合蛋白1(NSEP1),是包含冷休克结构域(CSD)的蛋白质的成员。它由三个结构域组成:N末端被认为是反式激活调节结构域,高度保守的核酸结合结构域(CSD)和C末端结构域,参与蛋白质-蛋白质相互作用。
Immunogen
合成肽对应于与KLH偶联的人YB-1氨基酸307-324。在大鼠和小鼠YB-1蛋白中的相应序列是相同的。
Application
抗YB-1(C末端)抗体已用于
- 免疫印迹
- 免疫沉淀
- 免疫荧光
- 双重免疫染色
Biochem/physiol Actions
Y-box结合蛋白(YB-1)显示与Y-box启动子元件结合以调节基因表达。它在多种细胞功能中起着重要作用,例如转录和翻译调节,DNA修复,耐药性和应激反应。
抗YB-1(C末端)抗体对大鼠、小鼠和人类的YB-1具有特异性。该免疫肽特异性抑制免疫印迹法检测YB-1条带。
Physical form
溶于含有15 mM叠氮化钠的0.01M磷酸盐缓冲盐水(pH 7.4)溶液中。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
相关内容
Instructions
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Wei W J, et al.
Nucleic Acids Research, 40(17), 8622-8636 (2012)
Sabine Brandt et al.
Cells, 13(5) (2024-03-13)
Fibrosis is characterized by excessive extracellular matrix formation in solid organs, disrupting tissue architecture and function. The Y-box binding protein-1 (YB-1) regulates fibrosis-related genes (e.g., Col1a1, Mmp2, and Tgfβ1) and contributes significantly to disease progression. This study aims to identify
The Y-box binding protein 1 suppresses Alzheimer?s disease progression in two animal models
Bobkova N V, et al.
PLoS ONE, 10(9), e0138867-e0138867 (2015)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| Y0396-200UL | 04061838038319 |