GenElute^™ Yeast Total RNA Miniprep Kit

Sigma′s GenElute Yeast Total RNA Purification Kit provides a simple and convenient way to isolate total RNA from yeast. Each kit is composed of two separate boxes. One part contains the appropriate supplementary reagents needed to ensure the effective disruption of the specific cell types. The other part contains the components for RNA purification.
RNA purification for yeast involves silica binding in a microspin format, thereby eliminating the need for CsCl gradients, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform. Cells are lysed and homogenized in a guanidine thiocyanate-containing buffer to ensure thorough denaturing of macromolecules and inactivation of RNases. Addition of ethanol causes RNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove contaminants, RNA is eluted in 50-100 μl of Elution Solution. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.

For additional information, please see www.sigma-aldrich.com/totalrna.

RNA shorter than 200 nucleotides in length, such as tRNA, 5S rRNA, and 5.8S rRNA, is not recovered efficiently under the conditions used with this kit.

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