Protein Expression: A Practical Approach

Details of the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems are included such as cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes and prokaryotic cells. Cell-free systems of both eukaryotes and prokaryotes are described in addition to the prokaryotic systems that offer coupled transcription-translation.