RNA-Protein Interactions: A Practical Approach

The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for in-depth analysis of the structural basis of the interaction. It includes a number of procedures such as the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA-protein interactions in yeast and E. coli.