Short Protocols in Molecular Biology, 4th ed.

Providing descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition explores molecular biology in an easily accessible format. It is designed to provide quick access to step-by-step instructions for essential methods. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection reflect the methods currently used in leading research facilities. New topics include: Informatics for Molecular Biologists, Analysis of Protein Interactions, Epitope Tagging.