Genome Analysis: Laboratory Manual Series

Assuming only a basic knowledge of molecular biology, these manuals explain how to clone, manipulate, analyze, and sequence large segments of DNA, and relate sequence to phenotype variation. Techniques, written for human as well as other animal genomes, deal plainly with sources of failure and how to avoid them. Protocols are written by experts, often by those who created the methods. The series is an excellent complement to the bible of recombinant DNA, Molecular Cloning.

This volume presents exhaustive coverage of techniques for: purifying genomic DNA; manipulating high-molecular weight DNA; several specialized DNA sequencing methods; and computational analysis of DNA and protein sequences. Appendices include recipes for common reagents, basic procedures, safety, and a compilation of ″useful facts.″