DNA Sequencing: Optimizing the Process and Analysis

DNA sequencing techniques have evolved rapidly in recent years, but scientists still encounter challenges in sequencing genomes. This book was written to help troubleshoot these difficulties while also providing a practical guide for those routinely sequencing DNA to refine and enhance their operation. This volume covers important topics in the field, including: biochemical and technological advances induced by the Human Genome Project; proven and newly emerging methods of preparing DNA templates; effects of some widely used laboratory reagents on DNA sequencing; Laboratory Information Management Systems (LIMS); and the future of DNA sequencing.