Anti-LRP6 Antibody, clone 1D11 ZooMAb^® Rabbit Monoclonal

Quality Control Testing

Evaluated by Western Blotting with FLAG^®-tagged recombinant Human LRP6 protein.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected FLAG^®-tagged recombinant Human LRP6 protein.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LRP6 in human kidney tissue sections.

Enzyme Immunoassay Analysis: Various dilutions of this antibody detected recombinant human LRP6.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D11 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Low-density lipoprotein receptor-related protein 6 (LRP6). It targets an epitope within 23 amino acids from the C-terminal, cytoplasmic domain.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Low-density lipoprotein receptor-related protein 6 (UniProt: O75581; also known as LRP-6) is encoded by the LRP6 gene (Gene ID: 4040) in human. LRP-6 is a disulfide-linked homodimeric, single-pass type I transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-1370), a transmembrane domain (aa 1371-1393), and a cytoplasmic tail (aa 1394-1613). It is a component of the Wnt-Fzd-LRP5-LRP6 complex that triggers β-catenin signaling. LRP-6 is widely co-expressed with LRP5 during embryogenesis and in adult tissues. Its levels decrease upon Wnt3A stimulation. LRP-6 contains five PPPSP motifs that play a central role in signal transduction following phosphorylation and activation of Wnt signaling pathway. Dual phosphorylation of cytoplasmic PPPSP motifs by GSK3 and CK1 is shown to be essential for AXIN1-binding, and subsequent stabilization and activation of β-catenin via preventing GSK3-mediated phosphorylation of β-catenin. Upon Wnt signaling, LRP-6 undergoes a cycle of caveolin- or clathrin-mediated endocytosis and plasma membrane location and it undergoes phosphorylation, aggregation, and co-localization with AXIN1 and GSK-3β at the plasma membrane in LRP-6 signalosomes. It is released from the endoplasmic reticulum following palmitoylation. However, in the absence of palmitoylation mono-ubiquitination retains it in the endoplasmic reticulum. LRP-6 is phosphorylated on serine 1490 by CDK14 during G2/M phase leads to regulation of the Wnt signaling pathway during the cell cycle. It is phosphorylated on threonine 1479 by casein kinase I (CK1, which is y TMEM198. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liang, J., et al. (2011). Mol. Cell Biol. 31(13); 2577-2590; Piao, S., et al. (2008). PLoS One. 3(12); e4046; Zheng, X., et al. (2005). Nature. 438(7069); 873-877).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, cytoplasmic region of human Low-density lipoprotein receptor-related protein 6 (LRP6).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany