羊毛塞通常用于GC进样口衬管中，以改善样品蒸发，和/或防止非挥发性物质进入色谱柱。它们还可用于填充的气相色谱柱、溶剂解吸管、热解吸管、以及吹扫捕集阱，以保留吸附床。

Glass wool is made up of short glass fibres and is nonflammable in nature. It is widely considered as a thermal insulation material in ships, automobiles, air conditioning ducts and water supply pipes. Furthermore, it is also suitable as noise absorbing material in concert halls, studios and movie theatres. Above all that it is non carcinogenic to humans. Glass wool treated with phosphoric acid is highly advantageous as it prevents usage of more amount of glass wool for metal column tubing. Moreover phosphoric acid is the most effective acid additive which is generally required in the stationary phase of the chromatographic column for deactivation of both the support and metal column tubing that is the glass wool. Glass wool treated with phosphoric acid is highly suitable for the analysis of free fatty acids (FFA).

Glass wool column maybe used for the separation of lymphocytes in human blood.

Phosphoric Acid Treated for acidic compounds such as barbiturates, free fatty acids, etc.