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Merck
CN

581325-U

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

别名:

Ascentis RP18 HPLC色谱柱

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关于此项目

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
25 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
C18 (octadecyl) phase
Pore size:
100 Å
Matrix:
fully porous particle, silica gel high purity, spherical
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产品名称

Ascentis ® C18 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Quality Level

material

stainless steel column

agency

suitable for USP L1 (Similar to Phenomenex Luna C18)

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

25% Carbon loading

parameter

≤70 °C temp. range, 400 bar pressure (5801 psi)

technique(s)

HPLC: suitable, LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

surface area

450 m2/g

surface coverage

3.7 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle, silica gel high purity, spherical

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质,孔径 100Å,硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用,柱内径可从微径柱(1.0mm 内径)扩展到制备尺寸(50mm 内径)。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相(反相酰胺)。

Ascentis C18 是特别稳定可靠的首选 HPLC 色谱柱,即使对于难以分离的化合物也能提供对称的峰形和出色的保留性能。

Application


  • Identification of recombinant human insulin and biosynthetic insulin analogues by multiplexed targeted unlabeled mass spectrometry of proteotypic tryptic peptides.: 本研究利用 Ascentis® C18 HPLC 色谱柱分离蛋白型胰蛋白酶肽,以鉴定胰岛素类似物,重点介绍了其在靶向质谱法进行蛋白质组学分析中的应用(Qasem RJ et al., 2019).。

  • Development and validation of a novel stability-indicating HPLC method for the simultaneous assay of betamethasone-17-valerate, fusidic acid, potassium sorbate, methylparaben and propylparaben in a topical cream preparation.: 本研究开发并验证了一种使用 Ascentis® C18 HPLC 色谱柱同时测定多种化合物的 HPLC 方法,证明了其在药物制剂稳定性测试中的稳健性(Byrne J et al., 2014)。

  • HPLC-DAD and HPLC-ESI-MS/MS methods for metabolite profiling of propolis extracts.: 本研究采用 Ascentis® C18 HPLC 色谱柱,使用 HPLC-DAD 和 HPLC-ESI-MS/MS 对蜂胶提取物代谢物进行分析,证明了其在复杂天然产物分析中的有效性 (Pellati F et al., 2011)。

  • Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.: 本文比较了不同的 HPLC 色谱柱技术,包括 Ascentis® C18,重点介绍了其在制药工艺开发中至关重要的快速分离方面的性能 (Abrahim A et al., 2010)。

Features and Benefits

  • 出色的保留性能
  • 优良的峰形对称性
  • 重现性好
  • 与 LC-MS 完全兼容

Other Notes

探索最适于 HPLCLC-MS 分析的 LiChropur 试剂

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany


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存储类别

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

相关内容

General Care and Use of Supelco™ HPLC Columns


Michael Kühl et al.
eLife, 9 (2020-01-22)
Far-red absorbing chlorophylls are constitutively present as chlorophyll (Chl) d in the cyanobacterium Acaryochloris marina, or dynamically expressed by synthesis of Chl f, red-shifted phycobiliproteins and minor amounts of Chl d via far-red light photoacclimation in a range of cyanobacteria
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis



全球贸易项目编号

货号GTIN
581325-U04061838548757