Product Name
Ascentis® Phenyl 高效液相色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
物料
stainless steel column
Agency
suitable for USP L11
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
19% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
15 cm × 4.6 mm
表面积
450 m2/g
表面覆盖度
5.2 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
phenyl phase
粒径
5 μm
孔径
100 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
应用
- A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison: This study highlights the advanced chromatographic techniques used to characterize pharmaceutical compounds, demonstrating the importance of accurate and detailed analysis in pharmaceutical research. The use of the Ascentis® Phenyl HPLC column facilitates high-resolution separations, essential for identifying degradation products in complex mixtures (Geven et al., 2023).
- HPLC with Spectrophotometric or Mass Spectrometric Detection for Quantifying Very-Long Chain Fatty Acids in Human Plasma and Its Association with Cardiac Risk Factors: This paper discusses the role of high-performance liquid chromatography (HPLC) in clinical settings, particularly for the assessment of biomarkers related to heart disease. Techniques involving the Ascentis® Phenyl HPLC column are employed to achieve precise and reliable quantification of fatty acids, critical for clinical diagnostics (Shrestha et al., 2021).
- A Fully Automated and Fast Method Using Direct Sample Injection Combined with Fused-Core Column On-Line SPE-HPLC for Determination of Ochratoxin A and Citrinin in Lager Beers: This study introduces an automated method for toxin analysis in beverages, utilizing the Ascentis® Phenyl HPLC column. The approach enhances sensitivity and throughput, showcasing the column′s efficacy in food safety applications (Lhotská et al., 2016).
- A Study of Retention Characteristics and Quality Control of Nutraceuticals Containing Resveratrol and Polydatin Using Fused-Core Column Chromatography: The research focuses on nutraceuticals, where the Ascentis® Phenyl HPLC column′s selectivity plays a pivotal role in segregating and analyzing health-promoting compounds, thereby ensuring product quality and compliance with industry standards (Fibigr et al., 2016).
- An On-Line SPE-HPLC Method for Effective Sample Preconcentration and Determination of Fenoxycarb and Cis, Trans-Permethrin in Surface Waters: This publication illustrates the application of the Ascentis® Phenyl HPLC column in environmental analysis, particularly for the sensitive detection of pesticides in water bodies. The column′s capabilities help in tracing low-level contaminants, critical for environmental monitoring and protection (Šatínský et al., 2015).
特点和优势
- 100% 水性兼容
- ANP/HILIC 和反相
- 梯度条件下的低紫外/质谱流失
- 交替选择性
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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