产品名称
Ascentis® Phenyl 高效液相色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L11
产品线
Ascentis®
特点
endcapped
制造商/商品名称
Ascentis®
包装
1 ea of
标记范围
19% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
15 cm × 4.6 mm
表面积
450 m2/g
表面覆盖度
5.2 μmol/m2
杂质
<5 ppm metals
基质
fully porous particle
silica gel high purity, spherical
基质活性基团
phenyl phase
粒径
5 μm
孔径
100 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
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一般描述
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
Ascentis Phenyl 苯基柱在反相模式（包括 100% 水性条件）下具有出色的分离能力。也可用于 HILIC/ANP（水性正相）模式，并在梯度应用时紫外/质谱流失低。
应用
- A Different Perspective on the Characterization of a New Degradation Product of Flibanserin With HPLC-DAD-ESI-IT-TOF-MSn and Its Pharmaceutical Formulation Analysis With Inter-Laboratory Comparison: This study highlights the advanced chromatographic techniques used to characterize pharmaceutical compounds, demonstrating the importance of accurate and detailed analysis in pharmaceutical research. The use of the Ascentis® Phenyl HPLC column facilitates high-resolution separations, essential for identifying degradation products in complex mixtures (Geven et al., 2023).
- HPLC with Spectrophotometric or Mass Spectrometric Detection for Quantifying Very-Long Chain Fatty Acids in Human Plasma and Its Association with Cardiac Risk Factors: This paper discusses the role of high-performance liquid chromatography (HPLC) in clinical settings, particularly for the assessment of biomarkers related to heart disease. Techniques involving the Ascentis® Phenyl HPLC column are employed to achieve precise and reliable quantification of fatty acids, critical for clinical diagnostics (Shrestha et al., 2021).
- A Fully Automated and Fast Method Using Direct Sample Injection Combined with Fused-Core Column On-Line SPE-HPLC for Determination of Ochratoxin A and Citrinin in Lager Beers: This study introduces an automated method for toxin analysis in beverages, utilizing the Ascentis® Phenyl HPLC column. The approach enhances sensitivity and throughput, showcasing the column′s efficacy in food safety applications (Lhotská et al., 2016).
- A Study of Retention Characteristics and Quality Control of Nutraceuticals Containing Resveratrol and Polydatin Using Fused-Core Column Chromatography: The research focuses on nutraceuticals, where the Ascentis® Phenyl HPLC column′s selectivity plays a pivotal role in segregating and analyzing health-promoting compounds, thereby ensuring product quality and compliance with industry standards (Fibigr et al., 2016).
- An On-Line SPE-HPLC Method for Effective Sample Preconcentration and Determination of Fenoxycarb and Cis, Trans-Permethrin in Surface Waters: This publication illustrates the application of the Ascentis® Phenyl HPLC column in environmental analysis, particularly for the sensitive detection of pesticides in water bodies. The column′s capabilities help in tracing low-level contaminants, critical for environmental monitoring and protection (Šatínský et al., 2015).
特点和优势
- 100% 水性兼容
- ANP/HILIC 和反相
- 梯度条件下的低紫外/质谱流失
- 交替选择性
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Dalibor Šatínský et al.
Talanta, 142, 124-130 (2015-05-25)
A new on-line SPE-HPLC method using fused-core columns for on-line solid phase extraction and large volume sample injection for increasing the sensitivity of detection was developed for the determination of insecticides fenoxycarb and cis-, trans-permethrin in surface waters. The separation
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