产品名称
TOYOPEARL® DEAE-650M, phase DEAE (diethylaminoethyl), bottle of 250 mL, 65 μm particle size
description
DEAE-650M
form
slurry
feature
counter ion Cl-
packaging
bottle of 250 mL
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
methacrylate
matrix active group
DEAE (diethylaminoethyl) phase
particle size
65 μm
operating pH
2-10
capacity
0.10 meq/mL±0.02 meq/mL ion exchange capacity
30 g/L±5 adsorption capacity (BSA)
30 mg/mL±5 mg/mL binding capacity (bovine serum albumin)
compatibility
mode of use weak anion exchange chromatography
separation technique
anion exchange
Application
TOYOPEARL® DEAE-650M is used in separation media and ion exchange resins. TOYOPEARL® DEAE-650M has been used in haemophilia studies.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
General description
Toyopearl DEAE-650 resin is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSKgel DEAE-5PW resin should be used. Selectivity remains the same.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
Günter Iberer et al.
Journal of chromatography. A, 972(1), 115-129 (2002-10-25)
Preparative continuous annular chromatography, a method to separate proteins in a truly continuous manner, was investigated in an industrial environment. Plasma-derived clotting factor IX concentrate was used as model protein. Separation of vitronectin, a common impurity in commercial available factor
Masahiko Takanashi et al.
Journal of bioscience and bioengineering, 97(1), 78-81 (2005-10-20)
D(-)-3-hydroxybutyrate dehydrogenase (BDH; EC 1.1.1.30) from a poly(D(-)-3-hydroxybutyrate) (PHB) degrading bacterium, Acidovorax sp. SA1, was purified using Toyopearl DEAE-650M, red-Sepharose CL-4B, and Q Sepharose FF. The molecular mass of the enzyme was estimated as 27 kDa by SDS-PAGE and 110
P R Foster et al.
Vox sanguinis, 86(2), 92-99 (2004-03-17)
The risk of haemophiliacs contracting variant Creutzfeldt-Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate
Christine Machold et al.
Journal of biotechnology, 117(1), 83-97 (2005-04-16)
Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding
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