material
aluminum seal, polypropylene and polyethylene frit
product line
ToyoScreen®
analyte chemical class(es)
proteins
packaging
pkg of 6 × 1 mL
manufacturer/tradename
Tosoh 21374
parameter
≤50% organic solvent, 0.2-1 mL/min flow rate, 3 bar max. pressure, 4 mL/min max. flow rate
technique(s)
HPLC: suitable
L × I.D.
3 cm × 6.4 mm
matrix
hydroxylated methacrylic polymer
matrix active group
phenyl phase
particle size
65 μm
operating pH
1-13
capacity
40 g/L±10 adsorption capacity (lysozyme)
separation technique
hydrophobic interaction (HIC)
General description
Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Legal Information
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation
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