material
aluminum seal, polypropylene and polyethylene frit
product line
ToyoScreen®
packaging
pkg of 1 × 5 mL (6 grades)
manufacturer/tradename
Tosoh 21399
parameter
≤50% organic solvent, 1-5 mL/min flow rate, 20 mL/min max. flow rate, 3 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
3 cm × 14.6 mm
matrix
hydroxylated methacrylic polymer
matrix active group
mixed phase
pore size
65-100 μm particle size
operating pH
1-13
separation technique
hydrophobic interaction (HIC)
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General description
Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular TOYOPEARL® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
Features and Benefits
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Legal Information
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation
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