物料
aluminum seal
polypropylene and polyethylene frit
产品线
ToyoScreen®
特殊分析物
IgG
包装
pkg of 5 × 1 mL
制造商/商品名称
Tosoh 23430
参数
0.2-1 mL/min flow rate
3 bar max. pressure
4 mL/min max. flow rate
技术
HPLC: suitable
长度 × 内径
3 cm × 6.4 mm
基质
hydroxylated methacrylic polymer
基质活性基团
rProtein A phase
粒径
45 μm
工作pH值
2-13
容量
>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
>80 g/L adsorption capacity (IgG)(static)
分离技术
affinity
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一般描述
Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.
特点和优势
Clean in place with 0.1 M NaOH.
法律信息
ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation
法规信息
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