V900891
胶原酶 来源于溶组织梭菌
Type I, for general use, Vetec™, reagent grade, powder, ≥125 CDU/mg solid
别名:
梭菌肽酶 A
应用
来自溶组织梭菌（Clostridium histolyticum）的胶原酶，可用于从许多类型的动物组织中分离细胞。VETECI型胶原酶具有胶原酶、酪蛋白酶、梭菌蛋白酶和胰蛋白酶活性的原始平衡，建议用于上皮细胞、肝细胞、肺细胞和肾上腺原代细胞的分离。
生化/生理作用
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
分析说明
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
其他说明
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
法律信息
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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