V900893
胶原酶 来源于溶组织梭菌
Type IV, Vetec™, reagent grade, powder, ≥160 CDU/mg solid
别名:
梭菌肽酶 A
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化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
232-582-9
MDL number:
Specific activity:
≥160 CDU/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
grade
reagent grade
product line
Vetec™
form
powder
specific activity
≥160 CDU/mg solid
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
storage temp.
−20°C
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General description
来自溶组织梭菌（Clostridium histolyticum）的胶原酶是一种胶原分解酶。它与胶原蛋白样物质相互作用，将胶原蛋白降解成小分子肽。
Application
来自溶组织梭菌（Clostridium histolyticum）的胶原酶，可用于从许多类型的动物组织中分离细胞。VETEC IV型胶原酶具有较低的胰蛋白酶活性。它适用于消化胰腺中的胰岛细胞。
适用于制备用于测序的单细胞悬浮液。
适用于制备用于测序的单细胞悬浮液。
Biochem/physiol Actions
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
Analysis Note
还含有梭菌蛋白酶、中性蛋白酶和胰蛋白酶活性。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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