y Version: 18T4 Gene 32 Protein
sigma-aldrich.com 3
1. General Information
1.1. Contents
Vial / bottle Label Function / description Catalog number Content
1 T4 gene 32 protein • Protein solution
(Vial 3): Final concentration: 15 mM
dATP and dTTP labeling mix Mix 1 volume of a 2.5 mM dATP or dTTP solution with 15 volumes of double-
distilled water and 4 volumes of [α-32 P]dATP or [α-32 P]dTTP
Vial 3): Final concentration: 15 mM
dATP and dTTP labeling mix Mix 1 volume of a 2.5 mM dATP or dTTP solution with
15 volumes of double-distilled water and 4 volumes of [α-32 P]dATP or
[α-32 P]dTTP
Usage: Column may be used 100 times.
Components
Seppro Mouse LC10 Column 1 each
(Catalog Number S5699)
10× Dilution Buffer 3 × 200 ml
Tris-Buffered Saline (TBS) - 100 mM
Tris-HCl with 1.5 M NaCl
Usage: Column may be used 100 times.
Components
Seppro Rat LC10 Column 1 each
(Catalog Number S6074)
10× Dilution Buffer 3 × 200 ml
Tris-Buffered Saline (TBS) - 100 mM
Tris-HCl with 1.5 M NaCl
Column may be used 100 times.
2
Components
Seppro IgY 14 LC10 Column 1 each
(Catalog Number S5074)
10× Dilution Buffer 3 × 200 ml
Tris-Buffered Saline (TBS) - 100 mM
Vial 3): Final concentration: 15 mM
dATP and dTTP labeling mix Mix 1 volume of a 2.5 mM dATP or dTTP solution with
15 volumes of double-distilled water and 4 volumes of [α-32 P]dATP or
[α-32 P]dTTP
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be
removal of
the fitting on the injector or detector.
• Option 2: female M6/male 10-32 fitting to
female 10-32/female 10-32 fitting two-piece
adapter (Catalog Number 55068) – A two-piece
adapter can be