CN
101-37-1
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关键词:'101-37-1'
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The Effective Use of Protein Kinase Inhibitors
95
86 101 105 106 34 37 57 87 81 26 104 70 64 75 61 92 102 55 106 53 85 101
95 100 95 103 88 82 81 98
91 84 21 128 89 98
5-Bromo-3-indolyl b-D -galactopyranoside (B2904)
containing pTZ
18R strain was plated on LB amp+ plates. The JM 101
(ESC 157) strain was plated on LB amp- plates. The
plates were incubated at 37 °C for 24-48 hours. The ESC
206 containing pTZ 18R
Instruction Manual: siIMPORTER™ siRNA and Plasmid DNA Transfection Reagent
Cat. #64-101
siIMPORTER™ Transfection Reagent
12
2. Using a 1.6ml microcentrifuge tube, dilute the
siIMPORTER™ reagent into serum-free medium
that has been warmed to 37°C according
The Effective Use of Protein Kinase Inhibitors
95
86 101 105 106 34 37 57 87 81 26 104 70 64 75 61 92 102 55 106 53 85 101
95 100 95 103 88 82 81 98
91 84 21 128 89 98
Product Information Sheet-P4406
Technol., 68, 91-101 (2017)
4. Huettner, J. E., and Baughman, R. W., J.
Neurosci., 6(10), 3044-3060 (1986).
5. Hasegawa, M. et al., Nippon Heikatsukin Gakkai
Zasshi, 23(1), 35-46 (1987).
MilliTrace™ Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
pipette, slowly add dropwise 9 mL Complete ES Media with 15% FBS and LIF
(Catalog No. ES-101-B) (pre-warmed to 37°C) to the 15 mL conical tube. IMPORTANT: Do
not add the whole volume of media at once
MilliTrace™ Nanog GFP Reporter Mouse Embryonic Stem Cell Kit
pipette, slowly add dropwise 9 mL Complete ES Media with 15% FBS and LIF
(Catalog No. ES-101-B) (pre-warmed to 37°C) to the 15 mL conical tube. IMPORTANT: Do
not add the whole volume of media at once
Serum-Free Expansion of CD34 Umbilical Cord Blood Using Stemline Hematopoietic Stem Cell Expansion Medium
data not shown).
101 102 103 104
CD34-APC
10
4
10
3
10
2
10
1
101 102 103 104
10
4
10
3
10
2
10
1
101 102 103 104
10
4
10
3
Product Information Sheet - SE120150
and mix 10 µL of serum with
1.0 mL of Sample Diluent (101-fold dilution).
Note: Do not dilute the standards, they have
already been pre-diluted 101-fold.
2. Secure the desired number of coated wells
O9-1 Mouse Cranial Neural Crest Cell Line
Development 140(5): 1034-1044.
Materials Required But Not Supplied
1. Complete ES Cell Medium with 15% FBS and
LIF (Cat. No. ES-101-B)
2. Recombinant Human bFGF (Cat. No. GF003)
3. Matrigel™
CCMCL1 Mantle Cell Lymphoma Cell Line
100 101 102 103 104
CD20
Yellow Fluorescence (YEL-HLog)
Plot P03, gated on G1
100 101 102 103 104
CD45
Yellow Fluorescence (YEL-HLog)
Plot P03, gated on G1
100 101
O9-1 Mouse Cranial Neural Crest Cell
Development 140(5): 1034-1044.
Materials Required But Not Supplied
1. Complete ES Cell Medium with 15% FBS and
LIF (Cat. No. ES-101-B)
2. Recombinant Human bFGF (Cat. No. GF003)
3. Matrigel™
Quality Control Ranges: SARS-COV2 ANTI-RBD IgG ELISA KIT
or deionized water.
CONTROL LOT NUMBER EXPECTED RANGE UNITS
QC 1 6110-101 5.3 – 11 ng/mL
QC 2 6110-201 18 - 37 ng/mL
Product Information Sheet - BCK555IVFCL
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK488IVHTSS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK594IVFCS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVIMS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK488IVIML
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK555IVHTSS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVHTSS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK594IVIMM
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVIMM
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVHTSL
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK555IVFCM
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK555IVIML
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK594IVHTSL
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK488IVHTSL
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVFCL
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK555IVIMS
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
Product Information Sheet - BCK647IVIML
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
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