108-40-7 | Sigma-Aldrich

The Effective Use of Protein Kinase Inhibitors

104 14 1 7 60 87 1 101 97 68 120 93 80 66 100 90 79 102 76 85 80 95 109 107 122 18 6 7 0 104 103 27 52 41 97 99 82 94 62

Product Information Sheet - PEROX1

From Cell Cultures (~2 × 108 cells) Perform the procedure at 4 °C. All solutions and equipment should be cooled before use. Homogenize the samples using a 7 mL Dounce glass tissue grinder

LABORATORY NOTEBOOK ISSUANCE PAGE

0.889 65–67 –108 –17 0.55 7.6 212 1.407 Toluene 0.865 110.6 –93 4 0.59 2.4(25) 284 1.496 1,1,2–Trichlorotri#uoroethane 1.57 47–48 –35 — 0.69 — 231 1.358 2,2,4–Trimethylpentane 0.692 98–99 –107 –7 0.5 1.94

Lab Notebook Guidelines

0.889 65–67 –108 –17 0.55 7.6 212 1.407 Toluene 0.865 110.6 –93 4 0.59 2.4(25) 284 1.496 1,1,2–Trichlorotri#uoroethane 1.57 47–48 –35 — 0.69 — 231 1.358 2,2,4–Trimethylpentane 0.692 98–99 –107 –7 0.5 1.94

Quality Control Ranges: Milliplex Human High Sensitivity T Cell Magnetic Bead Panel

IL-10 Control 1 52 – 108 pg/mL IL-6 Control 1 5.7 – 12 pg/mL Control 2 254 – 527 pg/mL Control 2 29 – 59 pg/mL

LABORATORY NOTEBOOK ISSUANCE PAGE

0.889 65–67 –108 –17 0.55 7.6 212 1.407 Toluene 0.865 110.6 –93 4 0.59 2.4(25) 284 1.496 1,1,2–Trichlorotri#uoroethane 1.57 47–48 –35 — 0.69 — 231 1.358 2,2,4–Trimethylpentane 0.692 98–99 –107 –7 0.5 1.94

The Effective Use of Protein Kinase Inhibitors

104 14 1 7 60 87 1 101 97 68 120 93 80 66 100 90 79 102 76 85 80 95 109 107 122 18 6 7 0 104 103 27 52 41 97 99 82 94 62

LABORATORY NOTEBOOK ISSUANCE PAGE

0.889 65–67 –108 –17 0.55 7.6 212 1.407 Toluene 0.865 110.6 –93 4 0.59 2.4(25) 284 1.496 1,1,2–Trichlorotri#uoroethane 1.57 47–48 –35 — 0.69 — 231 1.358 2,2,4–Trimethylpentane 0.692 98–99 –107 –7 0.5 1.94

Data Sheet - B7150 - Lot 061K88041

Electron Ionization Mass Spectrum 40 50 60 70 80 90 100 110 120 130 140 150 160 0 5000 15000 m/z Abundance 41 52 65 74 81 92

Product Information Sheet-LYSISO1

Dilution Buffer: 3.1. For tissue extracts from 4 g of tissue: 40 mL 3.2. For cell culture extracts from at least 2 × 108 cells: 30 mL OptiPrep™ Density Gradient Medium Solutions

Data Sheet - B7150 - Lot 61K8804

Spectrum 40 50 60 70 80 90 100 110 120 130 140 150 160 0 20000 40000 60000 80000 100000 120000 m/z Abundance 41 46 52

Product Information Sheet - RPROTKRO

0.2–0.5 cm (25–50 mg) mouse tail • 109 bacteria or 108 yeast cells • 25–50 mg formalin-fixed paraffin-embedded tissue section 0519.03368548001➆ Add 40 �l of the reconstituted proteinase K solution to

Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium

06 6.00E+06 0 1 2 3 4 5 6 7 8 9 Days in Culture V ia bl e Ce ll De ns ity (C el ls /m L) (S ol id

Data Sheet - S5174

(1994) 14. Jendraschak, E. and E.H. Sage, Semin. Cancer Biol., 7, 139 (1996) 15. Leddda, R. et al., J. Invest. Dermatol. 108, 210 (1997) 16. Strandjord, T.P. et al., Am. J. Respir. Cell Mol. Biol

111499 Microbiology Geobacillus stearothermophilus Spore suspension

Geobacillus stearothermophilus spore suspension, adjusted to a con- centration of 108 KBE/ml (stray range: 7 · 107 to 3 · 108 KBE/ml). Test agar for the antibiotics residue test according to KUNDRAT, Cat.

Validation and Qualification for Durapore® Sterilizing-Grade (0.22 µm) Membrane VMF4

16.0 8.22 96 21 10.2 4.30 118 7 13.6 >8.29 106 20 10.3 4.35 113 10 14.1 >8.29 99 54 12.6 6.22 108 11 13.8 >8.29 93 22 12.4 6.25 104 12 13.6 >8.29 89

Product Information Sheet-rprotk-ro

0.2 – 0.5 cm (25 – 50 mg) Bacteria 1 × 109 Yeast cells 1 × 108 Formalin-fixed, paraffin-embedded tissue sections 25 – 50 mg Add 40 μl of the reconstituted Proteinase K solution from

Product Information Sheet-rprotksol-ro

0.2 – 0.5 cm (25 – 50 mg) Bacteria 1 × 109 Yeast cells 1 × 108 Formalin-fixed, paraffin-embedded tissue sections 25 – 50 mg Add 40 μl of the Proteinase K solution from Step

Product Information Sheet - A7410

J. Physiol., 242(3), C131-C145 (1982). 7. Avery, R. L., et al., Systemic amiloride inhibits experimentally induced neovascularization. Arch. Ophthalmol., 108(10), 1474-1476 (1990). FEB/RXR 11/02

Complement C5 Deficient Serum (C1163)

Erythrocytes (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30 200 270–v

Complement C2 Deficient Serum (C0913)

EA7S (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30 200 270–v

A New Vacuum-Based Method for Adenovirus Purification and Concentration

Syringe Filter 7 min. 12 sec. n/a 4 min. 6 sec. 2 min. 5 min. 25 sec. 15 min. 40 sec. Supplier Y Syringe Filter 2 min. 30 sec. 2 min. 3 sec. 4 min. 4 min. 30 sec.

Detection of Campylobacter spp. in Food with a New Test Kit System

measles virus mRNA from a crude cell lysate. Analytical Biochemistry. 189(1):40-50. https://doi.org/10.1016/0003-2697(90)90041-7 6. Humphrey T, O'Brien S, Madsen M. 2007. Campylobacters as zoonotic pathogens

N-TER/siRNA nanoparticle mediated knockdown of gene expression

NFSdilute 108 108 288 288 432 432 720 720 1260 1260 NFS 9 18 25 51 35 71 66 133 166 332 0.5´ N-TER Buffer 99 90 263 237 397 361 654 587 1094 928 Serum-free

Product Information Sheet-rdlig-ro

uncut vector DNA, such as 50 pg. Transformation efficiency of cells per microgram of DNA >1 × 108 . Transformation of linearized unligated vector DNA. Completeness of restriction enzyme cleavage <1%

Data Sheet - C9774 - Lot 076K8807

requirements Pass Specific rotation, [α]D25 5 mg/ml solution in alcohol between +105° and +112° +108° Assay  high pressure liquid chromatography not less than 97.0% and not more than 103.0%

Complement C7 Deficient Serum (C1413)

Erythrocytes (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30

Data Sheet - C8788

Erythrocytes (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30

Complement C4 deficient serum (C1038)

Erythrocytes (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30 200 270–v

Complement C6 deficient serum human (C1288)

erythrocytes (1.5 × 108 cells/ml) (µl) GVB2+ (µl) Distilled water (µl) A** v – 200 300–v – B v 5 200 295–v – C v 10 200 290–v – D v 20 200 280–v – E v 30 200 270–v – F v