samples. J. Appl. Physiol., 27, 120-2
(1969).
3. Parvin, R. et al., Convenient rapid determination of
picomole amounts of oxaloacetate and citrate.
Anal. Biochem., 104, 296-9 (1980).
Amicon is a registered
permanently affix the DNA to the membrane, bake
at 80°C for 30 minutes and/or irradiate the
membrane (DNA side toward the light source) with
120 mjoules of 254 nm ultraviolet light.
8. The membranes
To
permanently affix the DNA to the membrane, bake
at 80°C for 30 minutes and/or irradiate the
membrane (DNA side toward the light source) with
120 mjoules of 254 nm ultraviolet light.
8. The membranes
sealed at −80 °C. Long term stability of this
product is being evaluated. Initial studies indicate that
the receptor can retain its original specific activity for
several months when stored at −80 °C in its
Storage: Store tightly sealed at room temperature.
Solubility: Soluble in water (120 mg/ml) or ethanol (80 mg/ml).
Disposal: Dissolve or mix the material with a combustible solvent and burn in
for 30 minutes. Cover to avoid direct
light.
9. Repeat Steps 6 and 7.
10. Measure the required volume of Substrate Solution
(1 mL/strip or 120 L/well) and place in a separate
container.
the DNA to
the membrane, bake at 80°C for 30 minutes and/or
irradiate the membrane (DNA side toward the light
source) with 120 mjoules of 254 nm ultraviolet light.
9. The membranes can be stored at
the DNA to
the membrane, bake at 80°C for 30 minutes and/or
irradiate the membrane (DNA side toward the light
source) with 120 mjoules of 254 nm ultraviolet light.
9. The membranes can be stored at
the DNA to
the membrane, bake at 80°C for 30 minutes and/or
irradiate the membrane (DNA side toward the light
source) with 120 mjoules of 254 nm ultraviolet light.
9. The membranes can be stored at