2458 (1983).
2. Shakelford, D., et al., Immunol. Rev., 66, 133
(1982).
3. Spies, T., et al., Proc. Nat. Acad. Sci., 82, 5165
(1985).
4. Accolla, R. S., et al., Sem. Hematol., 21, 287
(1984).
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
bacterial mutations affecting the restriction and
modification of DNA. J Mol Biol.1966;16:118-133.
• Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression
).
Eisenbarth, G.S., et al., J. Immunol., 124, 1237 (1980).
Sutherland, D.R., et al., J. Immunol., 133, 327 (1984).
Aruffo, A. and B. Seed, EMBO J., 6, 3313 (1987).
1/98
Sigma brand products are
-PAGE Gel of Lot Number 019K1608:
>90% (densitometry)
BRK
37
75
100
150
250
50
Figure 2.
Specific Activity of Lot Number 019K1608:
133 nmole/min/mg
0
100,000
200,000
300,000
-PAGE Gel of Lot Number 020M0847:
>90% (densitometry)
BRK
37
75
100
150
250
50
Figure 2.
Specific Activity of Lot Number 020M0847:
133 nmole/min/mg
0
100,000
200,000
300,000
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
1753 (1947).
4. Globulins are precipitated in a heat step following addition of caprylate, then removed by filtration.
5. The Plasma Proteins, 2nd Ed., Vol. I, F. W. Putnam, Ed., pp. 133-181, Academic
column to the top with
buffer. Allow ~5 CV of buffer to drain through the
bed at a flow rate at least 133% of the flow rate
to be used in the procedure. The bed height
should have
The average bead diameter is ~90 µm. Oxidizing
agents, and anionic detergents and buffers, should
not be used with DEAE Sepharose® Fast Flow.
Prolonged exposure to pH 4 should also be avoided.
The
nucleus accumbens.
Immunohistochemistry: 4% paraformaldehyde fixed tissue (care should be taken not to
over-fix tissue); perfusion followed by less than 90 minutes post-fixation, then
cryoprotect.
nucleus accumbens.
Immunohistochemistry: 4% paraformaldehyde fixed tissue (care should be taken not to
over-fix tissue); perfusion followed by less than 90 minutes post-fixation, then
cryoprotect.
column to the top with
buffer. Allow ~5 CV of buffer to drain through the
bed at a flow rate at least 133% of the flow rate
to be used in the procedure. The bed height
should have
column to the top with
buffer. Allow ~5 CV of buffer to drain through the
bed at a flow rate at least 133% of the flow rate
to be used in the procedure. The bed height
should have
glycerol.
Molecular mass: ∼112 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 133–181 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not