Storage/Stability
Recombinant bovine eNOS should be stored at or
below −70 °C. The solution will be stable for at least 6
months at that temperature. The enzyme loses
approximately 10% of its activity
lyophilized material at -20°C to -70°C for up to 6 months after date of receipt. After
reconstitution maintain frozen at -20°C to -70°C in undiluted aliquots for up to 6 months.
Avoid repeated freeze/thaw
kDA.
The product is supplied in phosphate buffered saline,
pH 7.4, (15 mM sodium phosphate and 135 mM sodium
chloride.
Activity: C1 esterase inhibitor (C1EI) functional activity is
determined
7.4, (2 x 10 ml).
6. Add 1 ml/plate of (1x) boiling sample buffer and
scrape cells.
7. Boil sample for 5 min. at 95 °C.
8. Aliquot samples of cells extracts and store at
–70 °C.
Preparation
709-715 (1977).
5. Podack, E.R. et al., J. Immunol., 121(2), 484-490
(1978).
6. Kolb, W.P. et al., J. Exp. Med., 135(3), 549-566
(1972).
7. Kolb, W.P., and Müller-Eberhard, H.J., J. Exp.
Med
incubate at 70 °C for
5 minutes.
4. Cool reaction immediately on ice and spin down
any condensation by centrifugation.
5. Add 1 µL of WTA Library Synthesis Enzyme and
mix by pipetting.
6. Incubate
0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼135 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 73–99 nmole/min/mg (see Figure 2)
Precautions
0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼135 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 73–99 nmole/min/mg (see Figure 2)
Precautions
0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼135 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 73–99 nmole/min/mg (see Figure 2)
Precautions
0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA,
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼135 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 73–99 nmole/min/mg (see Figure 2)
Precautions
hazards
and safe handling practices.
Storage
The product should be stored at –70 C. It will remain
active for at least 6 months at that temperature. The
enzyme loses 10% of its activity after a single
.
Storage/Stability
Mouse iNOS should be stored at or below −70 °C. The
solution will be stable for at least 6 months at that
temperature. The enzyme loses approximately
Storage/Stability
Recombinant rat bNOS should be stored at or below
−70 °C. The solution will be stable for at least 6 months
at that temperature. The enzyme loses approximately
40% of its activity
sequence.
Anti-phospho-Phospholipase C γ-1 (pTyr783) recognizes
the endogenous form of PLC γ protein (135 kDa) when
phosphorylated on tyrosine 783 and does not cross-
react with a site-directed mutant (Y783F
glutathione,
0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25%
glycerol.
Molecular mass: ∼135 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 5.3–7.1 nmole/min/mg (see Figure 2)
mM NaCl, 150 mM imidazole, 0.1 mM PMSF,
0.2 mM DTT, and 25% glycerol.
Molecular mass: ∼135 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 717–971 nmole/min/mg (see Figure 2)
Precautions
TEC protein tyrosine kinase
inhibits CD25 expression in human T-lymphocytes.
Immunol. Lett., 127, 135-142 (2010).
PRECISIO is a registered trademark of Sigma-Aldrich
Co. LLC.
TD,MAM 11/11-1
thoroughly by vortexing the mixture.
3. Add 135 µL cell culture media into the ECM/Component mixture from step 2 to yield a final hydrogel concentration
of 6 mg/mL. Mix thoroughly by vortexing. Note: While
thoroughly by vortexing the mixture.
3. Add 135 µL cell culture media into the ECM/Component mixture from step 2 to yield a final hydrogel concentration
of 6 mg/mL. Mix thoroughly by vortexing. Note: While
7.4 (2 x 10 ml).
6. Add 1 ml/plate of (1x) boiling sample buffer and
scrape cells.
7. Boil sample for 5 min. at 95 °C.
8. Aliquot samples of cells extracts and store at
–70
thoroughly by vortexing the mixture.
3. Add 135 µL cell culture media into the ECM/Component mixture from step 2 to yield a final hydrogel concentration
of 6 mg/mL. Mix thoroughly by vortexing. Note: While
thoroughly by vortexing the mixture.
3. Add 135 µL cell culture media into the ECM/Component mixture from step 2 to yield a final hydrogel concentration
of 6 mg/mL. Mix thoroughly by vortexing. Note: While