be tested. The liquid media are tightly closed and incubated for 20 to 21 days at 35-37
°C.
On days 2-4, 6-8, 13-15, and 19-21 after inoculation the liquid media (article number 146180)
to be tested. The liquid media are tightly closed and incubated for 20 to 21 days at 35-37 °C.
On days 2-4, 6-8, 13-15, and 19-21 after inoculation the liquid media (article number 146180) are
subcultured
of 35
17. EPA, Subchapter D 40 CFR part 136 Guidelines Establishing Test Preoceedures for the
Analysis of Pollutants. 2001.
Merck KGaA, Darmstadt, Germany
, E. Schaafsma, A.
Huijsmans, H. Robben, G. Schaart and P. Vooijs. American Journal of Pathology
136:641-655, 1990
2. Immunohistochemical demonstration of Keratin 7 in routinely fixed paraffin-embedded
Prusiner, S. B., et al., Science, 216, 136-144
(1982).
4. Stahl, N., et al., Cell, 51, 229-240 (1987).
5. Caughey, B., et al., J. Virol., 63, 175-181 (1989).
6. Brown, D. R., Trends Neurosci., 24
00:00 00:05 00:10 00:15 00:20 00:25 00:30 00:35 00:40 00:45 00:50 00:55 01:00 01:05 01:10 01:15 01:20 01:25 01:30
time (mm:ss)
flow (l/min)
136
B
A c
The volume of air sampled is counted
results of ICR Swabs
S. aureus
(24)
S. aureus
(93)
B. subtilis
spores (6)
B. subtilis
spores (26)
E. coli
(136)
E. coli
(650)
P. aeruginosa
(32)
P. aeruginosa
(228)
3
1983)
3. Dawson, R.M.C. et al., eds. Data for Biochem. Res. 3rd edition, Clarendon Press, Oxford, 136, 1987.
4. Specifications and Criteria for Biochemical Compounds, 3rd edition, National Academy of
pelleted sample in 150 µL of PBS,
pH 7.5 and vortex thoroughly.
6. Add *5-20 µL of MetaPolyzyme to sample solution.
7. Incubate at 35 °C for 2-24 hours.
8. Follow your standard DNA extraction protocol
thoroughly.
6. Optional: if solution will sit for more than 4 hours,
sodium azide may be added to 0.02%.
7. Add 20 µL (*) of MetaPolyzyme, DNA free to
sample solution.
8. Incubate