Lysate (LAL) Test.
Sterilization May be steam sterilized 150 times (forward) and 50 times (reverse)
for 30 minutes at 145°C.
Quality Management System This product was manufactured in a facility whose
membrane suspension, at a receptor density
of ∼6 pmoles/mg protein. The membrane suspensions
are supplied at a protein concentration of ∼1 mg/mL in
50 mM Tris-HCl containing 10% sucrose, pH 7.4.
Precautions
Preparation Instructions
This product is soluble in water (50 mg/ml). It is
reported to be soluble up to 1g/ml in water at 25 °C.6 A
1% aqueous solution has a pH of approximately 10.5.
References
be assayed into
a well of the 96 well plate. Up to 50 µl of extract can be assayed in a single reaction to yield
linear results.
10. Add 145 µl of complete BetaRed Reaction Buffer (with DTT and
properties of guanylyl cyclase. It
increases Vmax from 0.1 to 14.5 µmol/min/mg (145 fold
increase), and lowers Km from 300 to 50 µM (6 fold
decrease).
A-350619 relaxes cavernosum tissue strips in a dose
Applications Ace Desig. Corning® Desig.
145–174 Coarse filtration, gas
dispersion
A EC(170–220)
70–100 Coarse filtration, gas
dispersion
B —
25–50 Filtration, gas
dispersion
C C(40–60
Applications Ace Desig. Corning® Desig.
145–174 Coarse filtration, gas
dispersion
A EC(170–220)
70–100 Coarse filtration, gas
dispersion
B —
25–50 Filtration, gas
dispersion
C C(40–60
Applications Ace Desig. Corning® Desig.
145–174 Coarse filtration, gas
dispersion
A EC(170–220)
70–100 Coarse filtration, gas
dispersion
B —
25–50 Filtration, gas
dispersion
C C(40–60
Applications Ace Desig. Corning® Desig.
145–174 Coarse filtration, gas
dispersion
A EC(170–220)
70–100 Coarse filtration, gas
dispersion
B —
25–50 Filtration, gas
dispersion
C C(40–60
Histamine Receptor 3 (HR3). By Western blot the antibody reacts with a band at ~ 50 kDa
on rat brain lysate. An additional band at ~ 145 kDa may be seen depending on sample
and antibody concentration used.
been
published.6
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses.
Preparation Instructions
This product is soluble in water (50 mg/ml), yielding
solubility assays involve only organic solvents:
10 mg/mL in methylene chloride, 6 mg/mL in
chloroform, 10 mg/mL in ethanol, 50 mg/mL in DMSO.
Solutions are clear, colorless to faint yellow. It is also
soluble
and
storing human liver microsomes on cytochrome
P450 activity. Arch. Biochem. Biophys., 331(2),
145-169 (1996).
LCM/MAM 3/04
Sigma brand products are sold through Sigma-Aldrich, Inc
in K562 cells has been studied.6 A cell culture study of
roscovitine on human tumor cell lines and a mouse
model of cancer has been reported.7 A study in
DU-145 prostate tumor spheroids of the action
Volume
Sampling Unit
5 5 Single Sampling Unit 6 E5SU461-90005
20 5 Single Sampling Unit 4 E5SU461-90020
NovaSeptum® GO High Purity Sampling Unit
50 5 Single Sampling Unit 8 E5SU711-10050
100
has been described.6
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or
other uses.
Preparation Instructions
This product is soluble in water (50 mg/ml), yielding a
gel and staining with
Coomassie shows only two main bands at 120 kDa (alpha5beta1 subunit) and 145 kDa (alpha5
subunit). A weak band at 205 kDa is due to dimerization of the alpha5beta1 subunit (Belkin
1), 141-145 (1996).
5. Jin, N., and Rhoades, R. A., Activation of tyrosine
kinases in H2O2-induced contraction in pulmonary
artery. Am. J. Physiol., 272(6 Pt 2), H2686-2692
(1997).
6. Hardin
, 32 Suppl B, 63-74 (1993).
6. Cox, S. K., et al., Determination of cefoxitin in
serum and tissue. J. Chromatogr. B Biomed. Sci.
Appl., 705(1), 145-148 (1998).
8-azaguanine-resistant hybridomas
suitable for the preparation of triomas. J.
Immunol. Methods, 145(1-2), 247-250 (1991).
5. Ramachandra, R. N., et al., Development and
characterization
University Press (Oxford, UK: 1993), pp. 79-83.
6. Kato, M. et al., J. Biol. Chem., 262, 7180 (1987).
7. Jufiwara, N. et al., Eur. J. Biochem., 143, 145
(1984).
DS,RBG,NDH,MAM 06/17-1