dilutions. A suggested dilution scheme is as
follows:
a) Label 7 test tubes #1-7 and “0 dose”. Add 950 L of the Sample Diluent to Standard tube #1.
Add 500 L of the Sample Diluent to Standard tubes #2
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2. Hamilton, A. J. and Baulcombe, D. C.,
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3. Tuschl, T., Zamore, P. D., Lehmann,
R., Bartel, D. P., and Sharp, P. A.
(1999) Genes and
K. Y.; Alsberg, E.; Damm, K. L.; Anderson, K. W.;
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(153) Yu
K. Y.; Alsberg, E.; Damm, K. L.; Anderson, K. W.;
Mooney, D. J. Biotechnol. Progr. 2001, 17, 945–950.
(152) Rowley, J. A.; Madlambayan, G.; Mooney, D. J. Biomaterials 1999, 20,
45–53.
(153) Yu
buffer, pH 3.0
Gradient with
flow gradient:
Initial composition: 0 – 8 min 55% A 45% B
isocratic, flow increases from 213 μL/min to
425 μL/min, 8.1 – 20 min 65% A 35% B isocratic,
flow 850 μL/min