Animal Cell Culture Vol 1. Alan R. Liss, Inc., New York, NY. pp. 215-230.
11. Yong, V. W. et al. (1988). Dev. Neuroscience 10:222-230.
20. Ruoslahti, E. and Pierschbacher, M. (1987). Science 238:491-497
260/230 ratios
appear to be
"too low."
In some cases, the 260/230 ratios may
be below the recommended range.
Downstream assays have not been shown to
be compromised by lower 260/230 ratios
260/230 ratios
appear to be
"too low."
In some cases, the 260/230 ratios may
be below the recommended range.
Downstream assays have not been shown to
be compromised by lower 260/230 ratios
Cancer Inst., 76, 1113
(1986).
9. Sugarman, B.J., et al., Science, 230, 943 (1985).
10. Shalaby, M., et al., J. Immunol., 135, 2060 (1985).
11. Bertolini, D R., Nature, 319, 516 (1986).
12. Dinarello
panspecific hemopoietin of
activated T lymphocytes (interleukin-3). Annu. Rev.
Immunol., 4, 205-230 (1986).
7. Davis G.E. et al., Control of vascular tube
morphogenesis and maturation in 3D extracellular
Sato, N. et al., JNCL, 78, 1113 (1986).
9. Sugarman, B. et al., Science, 230, 943 (1985).
10. Shalaby, M. et al., J. Immunol., 135, 2060 (1985).
11. Bertolini, D., Nature, 319, 516 (1986).
12. Dinarello
(I50=230 µM).
37
TLCK also inhibited human complement C6 (4 x 10-7M) in the range of 1O-2-1O-3M38, dog urinary kallikrein
(Ki=6.2 x 10
-3M)39, human enterokinase40, and boar acrosin (at 2 µM).41
Chemical Abstracts Registry data, American
Chemical Society.
2. Sigma Quality Control Data
3. Jager, A.K. et al., J. Chromatogr., 634, 135 (1993).
4. Christensen, S.B., and Schaumburg, K., J. Org.
µL.
2
Sample Preparation
Proteins in samples may impact background levels. To
remove proteins from samples, deproteinize using a
10 kDa MWCO spin filter.
Add 80–135 µL of the samples
M Ileum J-pouch Normal margin to UC SCC361
ht-227-D 62 M Duodenum Normal margin to UC SCC362
ht-230-D 59 F Duodenum Normal margin to UC SCC363
ht-206-CR 24 F Colon Crohns SCC365
ht-206-I-CR 24 F Ileum
Inhibitor Cocktail KA 1 ml 20-119 $94
PKC Substrate Cocktail KA 1 ml 20-131 $135
PP2A
Anti-Protein Phosphatase 2 A/C H M R B Ch WB Rb 100 µL AB1621 $265
P2A Immunoprecipitation
P. (1992)
Plasmid 27, 220–230.
2. Nijkamp, H. J. J., de Lang, R.,
Stuitje, A. R., van den Elzen P. J. M.,
Veltkamp, E., and van Putten, A. J.
(1986) Plasmid 16, 135–160.
3. Som, T. and Tomizawa
folded)
• Coarse particles
• Oil chemistry
• Fast filtration speed with high loading capacity
230 9.0 cm 50 WHA5230090
11.0 cm 50 WHA5230110
12.5 cm 50 WHA5230125
15.0 cm 50 WHA5230150