Animal Cell Culture Vol 1. Alan R. Liss, Inc., New York, NY. pp. 215-230.
11. Yong, V. W. et al. (1988). Dev. Neuroscience 10:222-230.
20. Ruoslahti, E. and Pierschbacher, M. (1987). Science 238:491-497
Voltage 230/400 V ~ 50 Hz
Number of Poles 2
Degree of Protection IP 65
Insulating Class CLF
Ventilation IC411 (TEFC)
Variable Speed Duty Quadratic Torque 5–50Hz
Thermostat 2 (PTC 110
260/230 ratios
appear to be
"too low."
In some cases, the 260/230 ratios may
be below the recommended range.
Downstream assays have not been shown to
be compromised by lower 260/230 ratios
260/230 ratios
appear to be
"too low."
In some cases, the 260/230 ratios may
be below the recommended range.
Downstream assays have not been shown to
be compromised by lower 260/230 ratios
Cancer Inst., 76, 1113
(1986).
9. Sugarman, B.J., et al., Science, 230, 943 (1985).
10. Shalaby, M., et al., J. Immunol., 135, 2060 (1985).
11. Bertolini, D R., Nature, 319, 516 (1986).
12. Dinarello
panspecific hemopoietin of
activated T lymphocytes (interleukin-3). Annu. Rev.
Immunol., 4, 205-230 (1986).
7. Davis G.E. et al., Control of vascular tube
morphogenesis and maturation in 3D extracellular
Sato, N. et al., JNCL, 78, 1113 (1986).
9. Sugarman, B. et al., Science, 230, 943 (1985).
10. Shalaby, M. et al., J. Immunol., 135, 2060 (1985).
11. Bertolini, D., Nature, 319, 516 (1986).
12. Dinarello
(I50=230 µM).
37
TLCK also inhibited human complement C6 (4 x 10-7M) in the range of 1O-2-1O-3M38, dog urinary kallikrein
(Ki=6.2 x 10
-3M)39, human enterokinase40, and boar acrosin (at 2 µM).41
12 mm 13 x 15 / 16 6.5 III (FL) 230/50-60/16 50 x 58 x 89 88 9 312 681 F81-HL
0.4-0.7 0.2-0.4 22-26 M16x1 8 / 12 mm 13 x 15 / 16 8 III (FL) 230/50/14 55
Chemical Abstracts Registry data, American
Chemical Society.
2. Sigma Quality Control Data
3. Jager, A.K. et al., J. Chromatogr., 634, 135 (1993).
4. Christensen, S.B., and Schaumburg, K., J. Org.
µL.
2
Sample Preparation
Proteins in samples may impact background levels. To
remove proteins from samples, deproteinize using a
10 kDa MWCO spin filter.
Add 80–135 µL of the samples
0,5 g 340 x 230 mm 80251093 276
TR3RS-M 3 kg 1 g 340 x 230 mm 80251156 324
TR6RS-EU2 6 kg 1 g 340 x 230 mm 80251094 276
TR6RS-M 6 kg 2 g 340 x 230 mm 80251157 324
TR15RS-EU2 15 kg
internal diameter
(i.d.) at three different flow rates, as shown in Table 2
below. In all cases, the feed tubing length was 230 cm;
this tubing length was chosen to represent a worst-case
tubing length
pathways simultaneously. HeLa:TNFα+CalA
(Cat. No. 47-230) and HepG2:Insulin (Cat. No. 47-227)
were used as stimulated cell lysates to compare the
2-plex assay results with those of a customized 16-plex
concentration from 2-25 g/L. No differences in
retention were observed with increased filter fouling to
90% plugged (V90).
Study 1: mAb 02.1 at 20 g/L was diluted in buffer
[10 mM Citrate, 135 mM NaCl pH5
13Calbiochem • Biologics 32.3
Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2)
Liquid, Protein G purified. Immunogen used was a
synthetic peptide corresponding to amino acids 230-240
of human TOR. Recognizes the ~290 kDa