disrupt the nuclear lamina during
granule-mediated cytolysis. Proc. Natl. Acad. Sci.
USA, 9, 5746-5751 (2001).
RJM 08/01
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
with 0.2 mM 5,5’-Dithio-bis(2-Nitrobenzoic Acid) Solution
(ATI/DTNB)
(Prepare 50 ml in Reagent C using Acetylthiocholine Iodide, Sigma Prod. No. A-5751, and
5,5’-Dithio-bis(2-Nitrobenzoic Acid), Sigma Prod
with 0.2 mM 5,5’-Dithio-bis(2-Nitrobenzoic Acid) Solution
(ATI/DTNB)
(Prepare 50 ml in Reagent C using Acetylthiocholine Iodide, Sigma Prod. No. A-5751, and
5,5’-Dithio-bis(2-Nitrobenzoic Acid), Sigma Prod
desalted protein. Determine the exclusion
volume of the column using Blue Dextran (Product
Code D 5751). Then apply the neutralized TFMS
reaction mixture onto the column and elute with water.
The deglycosylated