cell
transglutaminase. Enzyme characterization and
regulation of activity. Biochem. J., 262, 633-641
(1989).
4. Facchiano, F., and Facchiano, A., Trans-
glutaminases and their substrates. Prog
purity) spotted on
Immobilon®-FL PVDF membranes in a 96-well format.
One membrane contains 46 histone H3 peptides (8 acetylated,
27 methylated, 5 phosphorylated, and 6 unmodified).
The other membrane
Cooper, MA, et al. The biology of human natural killer-cell subsets. TRENDS in Immunology.
22: 633 (2001).
2. Reefman, et al. Cytokine secretion is distinct from secretion of cytotoxic granules
1x Assay Buffer HSC.
10. Add 5 uL of 7-AAD reagent to each well.
11. Samples are now ready for acquisition on a flow cytometer equipped with a 488 nm and a 633
1205-1227 (1974).
5. Bernt, E. and Bergmeyer, H.-U., Methods of
Enzymatic Analysis, 2nd ed., pp. 2246-2248
(1974).
6. Näslund, B. et al., J. Lipid. Res., 34(4), 633-641
(1993).
7. Hayes
20 μl) 5 x 96 capillaries, containing 5 boxes, each with
96 capillaries and stoppers, 5 boxes, each with
96 capillaries and stoppers
04 929 292 001
LightCycler® 480 Multiwell Plate 96, white