https://www.sigmaaldrich.com
2. How to Use this Product
y Version: 05 Rapid DNA Dephos & Ligation Kit
8 sigma-aldrich.com
For the ligation, set up the following reaction:
Reagent
Inject 8-12 cells/C57BL/6 blastocyst.
Day 0
Prepare the following culture dishes containing a monolayer of mitotically inactivated PMEF
feeder cells:
• 1 x 25 cm2 flask
• 1 x 75
minutes.
Stop the reaction by adding 2 µl 0.2 M EDTA, pH 8.0 to a final concentration of 8 mM.
– Heat to +75°C for 10 minutes.
The concentration of EDTA must be considered for
Catalog # 05-429, 1 μg/mL).
Proteins were visualized using a
goat anti-mouse secondary
antibody conjugated to HRP and a
chemiluminescence detection
system.
250
150
100
75
Chem., 276, 4211
(2001).
16. Walter, J., et al., J. Biol. Chem., 276, 14634 (2001).
ER/KAA 05/05
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
Sigma's CHO Protein-Free Medium vs Competitors'
Cell Growth and Viability
0.0E+00
2.0E+05
4.0E+05
6.0E+05
8.0E+05
1.0E+06
1.2E+06
1.4E+06
1.6E+06
0 5 10 15 20
Days in Culture
Figure 1
V
ia
., 176, 7566-75
(2006).
5. Filova E., et al., Int J Nanomedicine, 10, 7145-63
(2015).
6. Fath KR., et al., J. Cell Sci., 92, 67-75 (1989).
7. Yan J., et al., Cell Mol Bioeng., 8, 151-59
beta-lactamases of Bacillus cereus.
Biochim. Biophys. Acta, 995, 264-272 (1989).
TMG,EM,NDH,ALF,MAM 05/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of
suitable for Protein Sequencing
Catalog Number P3428
Storage Temperature 2–8 °C
TECHNICAL BULLETIN
CAS RN 72561-05-8
EC 3.4.21.50
Synonym: Lys-C
Product Description
Endoproteinase Lys-C from
Testing Amino Acid Mix, Metals,
and Iron Mix 1
0.0E+00
5.0E+05
1.0E+06
1.5E+06
2.0E+06
2.5E+06
3.0E+06
3.5E+06
4.0E+06
4.5E+06
0 2 4 6 8 10 12 14
Days
V
ia
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le
C
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