suitable for Protein Sequencing
Catalog Number P3428
Storage Temperature 2–8 °C
TECHNICAL BULLETIN
CAS RN 72561-05-8
EC 3.4.21.50
Synonym: Lys-C
Product Description
Endoproteinase Lys-C from
of:
Dulbecco’s MEM with high glucose
8% FBS
1X NEAA
10 mM HEPES
100 units penicillin
0.1 mg/mL streptomycin
4 mM L-glutamine
b. Cultivate cells in T-75 flasks in a cell culture incubator set at
Carbon disulfide; 75-15-0] 및 이를 0.1% 이상 함유한 혼합물질
급성 흡입독성,
인화성 액체
2019-5 산화에틸렌[Ethylene oxide; 75-21-8] 및 이를 0.1% 이상 함유한 혼합물질
급성 흡입독성,
인화성 가스
2019-6 메틸디클로로실란[Methyldichlorosilane ; 75-54-7] 및 이를 10%
use in diagnostic procedures.
IFU-HAGP1SF-12K Rev 05/23 5
Storage Conditions Upon Receipt
• Recommended storage for kit components is 2-8 °C.
• For long-term storage, freeze reconstituted standards
For research use only. Not for use in diagnostic procedures.
48-619MAG Rev 05/23 8
Technical Guidelines
To obtain reliable and reproducible results, the operator should
Testing Amino Acid Mix, Metals,
and Iron Mix 1
0.0E+00
5.0E+05
1.0E+06
1.5E+06
2.0E+06
2.5E+06
3.0E+06
3.5E+06
4.0E+06
4.5E+06
0 2 4 6 8 10 12 14
Days
V
ia
b
le
C
el
ls
p
er
m
Sheath Fluid in each well and 75 μL should be aspirated.
• For CSF samples that require dilution, use the Assay Buffer.
HNS1MAG-95K Rev. 05-FEB-2021 PAGE
Ro 09-0198, an
immunopotentiating peptide. Tetrahedron Lett., 29,
4771-4772 (1988).
KAA,DWF,MAM 05/12-1
2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
Safety Data Sheet
for information regarding hazards and safe
handling practices.
N0186dat Rev 05/21
Preparation Instructions
This material is non-sterile. Prepare a stock solution
of 200
1995).
6. Jefferson, R.A., et al., Proc. Natl. Acad. Sci., 83,
8447-8451 (1986).
AC,MAM 03/05-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants
For research use only. Not for use in diagnostic procedures.
MSHMAG-21K Rev 05/23 8
• If samples fall outside the dynamic range of the assay, further dilute the samples
5′pAGCTTNNN
NNNTCTAGp5′
Director™ Universal Cloning System Manual
8 Version Date 10-05-05
of the template. However, other techniques such as using “touchdown” PCR may further