CN
90-96-0
应用筛选条件
关键词:'90-96-0'
显示 1-30 共 997 条结果 关于 "90-96-0" 范围 技术文档
The Effective Use of Protein Kinase Inhibitors
6 72 36 93 84 94 14 26 90 101 82 61
48 75 38 99 86 8 6 54 66 10 83 105 106 103
99 98 103 104 101 67 95 77 101 95 82 99
The Effective Use of Protein Kinase Inhibitors
93 111 95 75 100 85 2 0 86 98 138 93 108 102 84 88 104
SAPK2b/p38β2 97 107 97 127 98 90 119 95 10 3 74 98 150 88 96 104 97 92 115
SAPK3/p38γ
Product Information Sheet - CS0012
µL 20 µL from 1 mM ATP solution
2 90 µL 10 µL from Tube 1
3 90 µL 10 µL from Tube 2
4 90 µL 10 µL from Tube 3
Product Information Sheet - MAK494
(µM)
1 10 µL 90 µL 50
2 6 µL 94 µL 30
3 3 µL 97 µL 15
4 0 µL 100 µL 0
3.
Product Information Sheet - MAK497
H2O2
(µM)
1 Standard
#1
10 µL 90 µL 40
2 Standard
#2
10 µL 90 µL 24
3 Standard
#3
Product Information Sheet - MAK513
Maltose
(µM)
1
10 µL of
500 µM Std
90 µL 50
2
10 µL of
300 µM Std
90 µL 30
3
10 µL of
Product Information Sheet - MAK500
Alanine
(µM)
1
10 µL of 200
µM Std
90 µL 20
2
10 µL of 120
µM Std
90 µL 12
3
10 µL of 60
Product Information Sheet - MAK537
Acid
(µM)
1
10 µL of 500
µM Std
90 µL 50
2
10 µL of 300
µM Std
90 µL 30
3
10 µL of 150
Product Information Sheet - MAK310
Detection
Add 0, 25, 50, and 100 L of the 1 mM NH4Cl
Standard Solution into wells of a 96 well plate. Add
ultrapure water to each well to bring the volume to
100 L, generating
Product Information Sheet - MAK120
100 µL 0 µL 500 µM
2 80 µL 20 µL 400 µM
3 60 µL 40 µL 300 µM
4 40 µL 60 µL 200 µM
5 30 µL 70 µL 150 µM
6 20 µL 80 µL 100 µM
7 10 µL 90 µL
Product Information Sheet - MAK505
1000 µM
Std
90 µL 100
2
10 µL of 600 µM
Std
90 µL 60
3
10 µL of 300 µM
Std
90 µL 30
4 -
Product Information Sheet - MAK508
100 µM
Std
90 µL 10
2
10 µL of 60 µM
Std
90 µL 6
3
10 µL of 30 µM
Std
90 µL 3
4 -
MultiScreen Ultracel-10 filter plate: Protein retention, recovery, volume recovery and guidelines for concentrating and desalting
3. Percent retentate starting from 0.5 mg/mL Cytochrome c.
0
20
40
60
80
90
100
70
50
30
10
Wells 1 – 96
%
R
Product Information Sheet - MAK332
(M)
1 100 L 0 L 500
2 80 L 20 L 400
3 60 L 40 L 300
4 40 L 60 L 200
5 30 L 70 L
Product Information Sheet - MAK501
H2O2
(µM)
1 Standard
#1
10 µL 90 µL 20
2 Standard
#2
10 µL 90 µL 12
3 Standard
#3
Product Information Sheet - MAK120
1 100 µL 0 µL 500 µM
2 80 µL 20 µL 400 µM
3 60 µL 40 µL 300 µM
4 40 µL 60 µL 200 µM
5 30 µL 70
Product Information Sheet - MAK504
Coenzyme A
(µM)
1
10 µL of 1000
µM Std
90 µL 100
2
10 µL of 600
µM Std
90 µL 60
3
10 µL of 300
Product Information Sheet - MAK368
10 L
of the Starch Standard (2.0 mg/mL) to 90 L of
ultrapure water, mix well. Prepare Starch Standards in
desired wells of a clear flat-bottom 96 well plate
according to Table 1.
Table 1.
Preparation
Product Information Sheet - MAK137
working solution.
Add 0, 30, 60, and 100 µL of the 300 µM Calibrator
solution into tubes. Add water to each tube to bring the
volume up to 100 µL, generating 0 (blank), 90, 180, and
Product Information Sheet - MAK395
Chromophore
(nmol/well)
1 0 L 100 L 0
2 2 L 98 L 2
3 4 L 96 L 4
4 6 L 94 L 6
5 8 L 92 L 8
6 10 L 90 L 10
Product Information Sheet - MAK484
alanine (µM)
1 90 µL 0 µL 300
2 60 µL 30 µL 200
3 30 µL 60 µL 100
4 0 µL 90 µL 0
3.
Product Information Sheet - MAK276
activity.
Standard Curve Preparation
Add 0, 20, 40, 60, 80, and 100 L of 4-Nitrocatechol
Standard into a series of wells in a 96 well plate to
generate 0, 10, 20, 30, 40, and 50 nmole/well of
Product Information Sheet - MAK329
(mM)
1 100 L 0 L 2.0
2 80 L 20 L 1.6
3 60 L 40 L 1.2
4 40 L 60 L 0.8
5 30 L 70 L
Product Information Sheet - MAK522
Reagent
Assay Buffer 90 µL 85 µL
Enzyme A 1 µL -
Enzyme B 1 µL 1 µL
Dye Reagent 1 µL 1 µL
Transfer 90
Product Information Sheet - MAK474
1 100 µL 0 µL 2.0
2 75 µL 25 µL 1.5
3 50 µL 50 µL 1.0
4 25 µL 75 µL 0.5
5 0 µL 100 µL 0
Product Information Sheet - MAK336
(mM)
1 100 L 0 L 2.0
2 80 L 20 L 1.6
3 60 L 40 L 1.2
4 40 L 60 L 0.8
5 30 L 70 L
Product Information Sheet - CASP8C
standard
solution
µl per well
1x Assay buffer
µl per well
0 0 100
1 5 95
2 10 90
5 25 75
10 50 50
15 75 25
Product Information Sheet - MAK531
. Transfer 10 µL Standards into separate wells of a
clear flat-bottom 96-well plate. Add 90 µL Assay
Buffer to the standards.
Detection Reagent Preparation
Prepare enough detection reagents
Product Information Sheet - MAK199
Solution with 90 L of water to prepare a
10 mM (10 nmole/L) AcAc Standard Solution. Add 0,
2, 4, 6, 8, and 10 L of the 10 mM AcAc Standard
Solution into a 96 well plate to generate 0
Product Information Sheet - MAK476
(µM)
1 200 µL 0 µL 300
2 120 µL 80 µL 180
3 60 µL 140 µL 90
4 - 200 µL 0
3. Mix well
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