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Lucrecia M Curto et al.
Biophysical journal, 103(9), 1929-1939 (2012-12-04)
Δ98Δ is a functional all-β sheet variant of intestinal fatty acid binding protein (IFABP) that was generated by controlled proteolysis. This framework is useful to study the molecular determinants related to aggregation of β-barrel proteins. Albeit displaying increased conformational plasticity
Thomas S Ream et al.
Molecular cell, 33(2), 192-203 (2008-12-27)
In addition to RNA polymerases I, II, and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play nonredundant roles in siRNA-directed DNA methylation and
Binbin Zhou et al.
Journal of inorganic biochemistry, 118, 68-73 (2012-11-06)
The intracellular α-synuclein (α-syn) protein, whose conformational change and aggregation have been closely linked to the pathology of Parkingson's disease (PD), is highly populated at the presynaptic termini and remains there in the α-helical conformation. In this study, circular dichroism
John P Hodkinson et al.
Rapid communications in mass spectrometry : RCM, 26(16), 1783-1792 (2012-07-11)
Amyloid formation is implicated in a number of human diseases. β(2)-Microglobulin (β(2)m) is the precursor protein in dialysis-related amyloidosis and it has been shown that partial, or more complete, unfolding is key to amyloid fibril formation in this pathology. Here
Samala Murali Mohan Reddy et al.
Organic & biomolecular chemistry, 12(32), 6181-6189 (2014-07-08)
Peptide based self-assembled structures, especially those from smaller peptides, have attracted much research interest due to their potential applications as biomaterials. These structures have been produced using different solvents (one of the methods), including alcohols, except fluorinated alcohols, which are
Gregory Cole et al.
Biochemistry, 51(25), 5033-5040 (2012-06-07)
Tetherin/Bst-2 is a cell surface protein that can act as a restriction factor against a number of enveloped viruses, including HIV-1. It acts by tethering new virus particles to the host cell membrane, promoting their internalization and degradation. Tetherin is
Applications of 2, 2, 2 trifluoroethanol as a versatile co-solvent in supercritical fluid chromatography for purification of unstable boronate esters, enhancing throughput, reducing epimerization, and for additive free purifications
Asokan K, et al.
Journal of Chromatography A, 1531, 122-130 (2018)
Bryce V Plapp et al.
Biochemistry, 51(19), 4035-4048 (2012-04-26)
Structures of horse liver alcohol dehydrogenase complexed with NAD(+) and unreactive substrate analogues, 2,2,2-trifluoroethanol or 2,3,4,5,6-pentafluorobenzyl alcohol, were determined at 100 K at 1.12 or 1.14 Å resolution, providing estimates of atomic positions with overall errors of ~0.02 Å, the
Anjali Jha et al.
Biophysical journal, 103(4), 797-806 (2012-09-06)
An important goal in studies of protein aggregation is to obtain an understanding of the structural diversity that is characteristic of amyloid fibril and protofibril structures at the molecular level. In this study, what to our knowledge are novel assays
Afshin Iram et al.
Archives of biochemistry and biophysics, 533(1-2), 69-78 (2013-03-19)
TFE induces structural alterations of proteins similar to the lipid environment of biological membranes, implicating these studies worthy of analyzing protein conformation in membranes such as red blood cells (RBCs). Heme loss occurs on rupturing of RBCs as found in
Britani N Blackstone et al.
Tissue engineering. Part A, 20(13-14), 1784-1793 (2014-01-15)
Recently, in vitro diagnostic tools have shifted focus toward personalized medicine by incorporating patient cells into traditional test beds. These cell-based platforms commonly utilize two-dimensional substrates that lack the ability to support three-dimensional cell structures seen in vivo. As monolayer
Paula M Ladwig et al.
Clinical chemistry, 60(8), 1080-1088 (2014-05-07)
Measurement of IgG subclasses is a useful tool for investigation of humoral immune deficiency in the presence of total IgG within reference intervals and IgG4-related disease. Nephelometry has been the method of choice for quantification. We describe an LC-MS/MS method
Carla R Angelani et al.
Biochimica et biophysica acta, 1844(9), 1599-1607 (2014-06-15)
Δ78Δ is a second generation functional all-β sheet variant of IFABP (intestinal fatty acid binding protein) corresponding to the fragment 29-106 of the parent protein. This protein and its predecessor, Δ98Δ (segment 29-126 of IFABP), were initially uncovered by controlled
Ryo Gotoh et al.
Molecules (Basel, Switzerland), 17(8), 9010-9022 (2012-08-01)
Focusing on the steric and electronic properties of the resonance-stabilized amidine framework, a cationic metal-bisamidine complex was designed as a conjugated combined Lewis-Brønsted acid catalyst. The chiral Zn(II)-bisamidine catalyst prepared from the 2,2'-bipyridyl derived bisamidine ligand, ZnCl₂, and AgSbF₆ promoted
The many roles for fluorine in medicinal chemistry.
William K Hagmann
Journal of medicinal chemistry, 51(15), 4359-4369 (2008-06-24)
Ellen M Green et al.
Biopolymers, 103(4), 187-202 (2014-11-05)
The cyanogen bromide-resistant proteins of lamprey cartilage are biochemically related to the mammalian elastic protein, elastin. This study investigates their mechanical properties and enquires whether, like elastin, long-range elasticity arises in them from a combination of entropic and hydrophobic mechanisms.
Kenichi Murai et al.
Organic & biomolecular chemistry, 11(16), 2648-2651 (2013-03-06)
CF3CH2OH was found to be a useful solvent for the oxidative rearrangement reactions of aminals promoted by N-chlorosuccinimide, which proceed via the intermediacy of in situ formed chloro-aminals and that produce 3,4-dihydroquinazolines.
Afshin Iram et al.
International journal of biological macromolecules, 50(4), 932-938 (2012-06-09)
A systematic investigation on the effects of trifluoroethanol and acetonitrile at various concentrations on cellulase (EC 3.2.1.4) was studied by enzyme assay, intrinsic fluorescence, ANS binding, circular dichroism and ATR-Fourier transform infra red spectroscopy. The results show the presence of
Jesper S Bahnsen et al.
Pharmaceutical research, 32(5), 1546-1556 (2015-03-18)
To investigate the suitability of three antimicrobial peptides (AMPs) as cell-penetrating antimicrobial peptides. Cellular uptake of three AMPs (PK-12-KKP, SA-3 and TPk) and a cell-penetrating peptide (penetratin), all 5(6)-carboxytetramethylrhodamine-labeled, were tested in HeLa WT cells and analyzed by flow cytometry
Malcolm E Tessensohn et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 16(1), 160-168 (2014-11-25)
Voltammetric experiments with 9,10-anthraquinone and 1,4-benzoquinone performed under controlled moisture conditions indicate that the hydrogen-bond strengths of alcohols in aprotic organic solvents can be differentiated by the electrochemical parameter ΔEp (red) =|Ep (red(1)) -Ep (red(2)) |, which is the potential
Eugénie Hébrard et al.
Virology journal, 6, 23-23 (2009-02-18)
VPgs are viral proteins linked to the 5' end of some viral genomes. Interactions between several VPgs and eukaryotic translation initiation factors eIF4Es are critical for plant infection. However, VPgs are not restricted to phytoviruses, being also involved in genome
Nico P M Smit et al.
Journal of proteomics, 109, 143-161 (2014-06-28)
In this study, we have followed up on previous liquid chromatography (LC) multiple reaction monitoring (MRM) mass spectrometry (MS) approaches for measurement of apolipoprotein (apo) A-I and apo B100 in serum aiming for implementation of a multiplexed assay in a
Xiqin Yang et al.
Methods in molecular biology (Clifton, N.J.), 970, 249-255 (2013-01-04)
Supercritical fluid chromatography is an environmentally benign separation technology due to the use of carbon dioxide modified with organic solvents as the mobile phase. It has found widespread use in the separation of chiral compounds based on the relative ease
Multiple aspects of the toxicity of fluroxene and its metabolite 2,2,2-trifluoroethanol.
L S Kaminsky et al.
Critical reviews in toxicology, 19(2), 87-112 (1988-01-01)
Young Min Shin et al.
Macromolecular bioscience, 14(8), 1190-1198 (2014-05-09)
Over the last decade, bone tissue engineering scaffolds have been advanced owing to the bioceramic incorporation and biomimetic modification. In this report, a dual-functional fibrous scaffold with a bioceramic and biomolecule is developed, and a combined effect of a dual-modification
Ganesh Shanmugam et al.
International journal of biological macromolecules, 54, 155-159 (2012-12-19)
Collagen, a fibrous structural protein, is a major component of skin, tendon, bone, and other connective tissues. Collagen is one of the dominant biomaterials used for tissue engineering and drug delivery applications. 2,2,2-Trifluoroethanol (TFE) has been used as a co-solvent
Michael Reinwarth et al.
Chembiochem : a European journal of chemical biology, 14(1), 137-146 (2012-12-12)
Bioactive peptides often contain several disulfide bonds that provide the main contribution to conformational rigidity and structural, thermal, or biological stability. Among them, cystine-knot peptides-commonly named "knottins"-make up a subclass with several thousand natural members. Hence, they are considered promising
Catherine Pan et al.
Journal of proteome research, 13(11), 4535-4545 (2014-05-24)
Despite extensive research, an unmet need remains for protein biomarkers of Parkinson's disease (PD) in peripheral body fluids, especially blood, which is easily accessible clinically. The discovery of such biomarkers is challenging, however, due to the enormous complexity and huge
Timo Glatter et al.
Molecular systems biology, 5, 237-237 (2009-01-22)
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP-MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP-MS analysis of
V L Anderson et al.
Physical biology, 9(5), 056005-056005 (2012-08-31)
Both increased temperature and moderate concentrations of fluorinated alcohols enhance aggregation of the Parkinson's disease-associated protein α-synuclein (αS). Here, we investigate the secondary structural rearrangements induced by heating and trifluoroethanol [TFE]. At low TFE concentrations, CD spectra feature a negative
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